Restriction map for
pF1KSDA0049
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflIII AgeI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BbrPI
BcnI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BssSI BstBI
BstEII BstPI BstUI Bsu36I CelII
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I Eco81I EcoO65I EcoT22I
FauI FspI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NarI NciI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1362 GTATAC GT^[AC][GT]AC
AcyI 1376 GGCGTC G[GA]^CG[CT]C
AflII 202 CTTAAG C^TTAAG
AhdI 1675 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 198 AGCGCT AGC^GCT
AspEI 1675 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1409 CTCGGG C^[CT]CG[GA]G
BalI 744 TGGCCA TGG^CCA
BamHI 387 GGATCC G^GATCC
BcgI 394 GCAGTGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 166 GTATCC GTATCC(6/5)
BfaI 304 CTAG C^TAG
BfrI 202 CTTAAG C^TTAAG
BglI 2755 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1376 GGCGTC G[GA]^CG[CT]C
BsaI 780 GAGACC GGTCTC(1/5)
BsmBI 475 GAGACG CGTCTC(1/5)
BsoBI 1409 CTCGGG C^[CT]CG[GA]G
BsrFI 1306 GCCGGC [GA]^CCGG[CT]
Bst1107I 1362 GTATAC GTA^TAC
BstXI 1395 CCAGCAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1362 GTATAC GTA^TAC
Cfr10I 1306 GCCGGC [GA]^CCGG[CT]
DsaI 1005 CCATGG C^C[GA][CT]GG
Eam1105I 1675 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 198 AGCGCT AGC^GCT
EcoNI 144 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 996 GAATTC G^AATTC
EcoRV 88 GATATC GAT^ATC
FseI 1305 GGCCGGCC GGCCGG^CC
HaeII 198 AGCGCT [GA]GCGC^[CT]
HapII 1307 CCGG C^CGG
HgaI 1377 GCGTC GACGC(5/10)
Hin1I 1376 GGCGTC G[GA]^CG[CT]C
HincII 496 GTTAAC GT[CT]^[GA]AC
HindII 496 GTTAAC GT[CT]^[GA]AC
HpaI 496 GTTAAC GTT^AAC
HpaII 1307 CCGG C^CGG
MaeI 304 CTAG C^TAG
MluNI 744 TGGCCA TGG^CCA
MscI 744 TGGCCA TGG^CCA
MspI 1307 CCGG C^CGG
NaeI 1306 GCCGGC GCC^GGC
NcoI 1005 CCATGG C^CATGG
NdeI 740 CATATG CA^TATG
NgoMIV 1306 GCCGGC G^CCGGC
NspI 1763 GCATGT [GA]CATG^[CT]
PshAI 2009 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2601 GAAGAGC GCTCTTC(1/4)
SbfI 2838 CCTGCAGG CCTGCA^GG
ScaI 821 AGTACT AGT^ACT
Sse8387I 2838 CCTGCAGG CCTGCA^GG
StuI 2274 AGGCCT AGG^CCT
TaqI 92 TCGA T^CGA
TthHB8I 92 TCGA T^CGA
XcmI 2225 CCAGCCGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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Asp700I 1525 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2854 GAACTTCTTC
BanI 254 GGCACC G^G[CT][GA]CC
2621 GGCACC
BbvCI 2086 GCTGAGG CCTCAGC(-5/-2)
2329 GCTGAGG
BclI 1481 TGATCA T^GATCA
2547 TGATCA
Bsp1407I 2249 TGTACA T^GTACA
2481 TGTACA
BsrGI 2249 TGTACA T^GTACA
2481 TGTACA
BstAPI 650 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
906 GCAAAGGTTGC
CfoI 199 GCGC GCG^C
2260 GCGC
DraI 31 TTTAAA TTT^AAA
1846 TTTAAA
EaeI 744 TGGCCA [CT]^GGCC[GA]
1308 CGGCCA
FbaI 1481 TGATCA T^GATCA
2547 TGATCA
HhaI 199 GCGC GCG^C
2260 GCGC
HinP1I 199 GCGC G^CGC
2260 GCGC
PpuMI 1673 AGGACCT [GA]G^G[AT]CC[CT]
1933 GGGACCC
SspBI 2249 TGTACA T^GTACA
2481 TGTACA
XmnI 1525 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2854 GAACTTCTTC
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII