Restriction map for
pF1KSDA0057
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HapII Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MflI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 560 AAATTC [GA]^AATT[CT]
AhdI 353 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 838 GATCC GGATC(4/5)
AocI 214 CCTAAGG CC^T[ACGT]AGG
ApoI 560 AAATTC [GA]^AATT[CT]
AspEI 353 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 220 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 841 CCCGAG C^[CT]CG[GA]G
AvaII 219 GGACC G^G[AT]CC
BalI 794 TGGCCA TGG^CCA
BbsI 445 GAAGAC GAAGAC(2/6)
BciVI 415 GGATAC GTATCC(6/5)
BfaI 692 CTAG C^TAG
BpuAI 445 GAAGAC GAAGAC(2/6)
BsaAI 397 TACGTG [CT]AC^GT[GA]
BsaI 190 GAGACC GGTCTC(1/5)
BsgI 514 CTGCAC GTGCAG(16/14)
BsmFI 991 GTCCC GGGAC(10/14)
BsoBI 841 CCCGAG C^[CT]CG[GA]G
BsrBI 31 CCGCTC CCGCTC(-3/-3)
BstAPI 872 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1044 CCATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 214 CCTAAGG CC^T[ACGT]AGG
CfoI 924 GCGC GCG^C
DraII 218 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1084 CCACGG C^C[GA][CT]GG
EaeI 794 TGGCCA [CT]^GGCC[GA]
Eam1105I 353 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 214 CCTAAGG CC^T[ACGT]AGG
EcoO109I 218 AGGACCT [GA]G^G[ACGT]CC[CT]
HaeII 923 GGCGCT [GA]GCGC^[CT]
HgaI 95 GCGTC GACGC(5/10)
HhaI 924 GCGC GCG^C
HinP1I 924 GCGC G^CGC
MaeI 692 CTAG C^TAG
MaeII 398 ACGT A^CGT
MluNI 794 TGGCCA TGG^CCA
MscI 794 TGGCCA TGG^CCA
NlaIV 1075 GGAACC GG[ACGT]^[ACGT]CC
PflFI 220 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 218 AGGACCT [GA]G^G[AT]CC[CT]
PstI 658 CTGCAG CTGCA^G
SapI 33 GCTCTTC GCTCTTC(1/4)
ScaI 662 AGTACT AGT^ACT
SmlI 1099 CTCAAG C^T[CT][GA]AG
SphI 264 GCATGC GCATG^C
StuI 908 AGGCCT AGG^CCT
TaqI 116 TCGA T^CGA
Tsp509I 561 AATT ^AATT
Tth111I 220 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 116 TCGA T^CGA
XcmI 430 CCAAGAAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 196 GTGCAC G^TGCAC
460 GTGCAC
AlwNI 735 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
873 CAGGCTCTG
ApaLI 196 GTGCAC G^TGCAC
460 GTGCAC
Asp700I 480 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
726 GAAACTGTTC
BpmI 574 CTCCAG CTGGAG(16/14)
608 CTGGAG
BsmAI 190 GAGAC GTCTC(1/5)
1104 GTCTC
BstEII 759 GGTTACC G^GT[ACGT]ACC
1039 GGTTACC
BstPI 759 GGTTACC G^GT[ACGT]ACC
1039 GGTTACC
DpnII 645 GATC ^GATC
838 GATC
EcoO65I 759 GGTTACC G^GT[ACGT]ACC
1039 GGTTACC
MboI 645 GATC ^GATC
838 GATC
MseI 421 TTAA T^TAA
622 TTAA
NdeII 645 GATC ^GATC
838 GATC
NspI 264 GCATGC [GA]CATG^[CT]
456 GCATGT
PleI 695 GACTC GAGTC(4/5)
989 GAGTC
Sau3AI 645 GATC ^GATC
838 GATC
SexAI 221 ACCTGGT A^CC[AT]GGT
593 ACCTGGT
SfcI 176 CTACAG C^T[GA][CT]AG
658 CTGCAG
TfiI 346 GAATC G^A[AT]TC
1033 GAATC
Tru9I 421 TTAA T^TAA
622 TTAA
XmnI 480 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
726 GAAACTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BglI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboII MnlI MslI MspA1I MvaI
MwoI NlaIII PvuII RsaI Sau96I
ScrFI TseI Tsp45I TspRI