Restriction map for
pF1KSDA0062
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflIII AgeI AhdI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BcgI BglI BlnI BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoT22I FauI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 320 CGCG CG^CG
AcyI 891 GGCGCC G[GA]^CG[CT]C
AflII 733 CTTAAG C^TTAAG
Alw44I 101 GTGCAC G^TGCAC
AocI 76 CCTGAGG CC^T[ACGT]AGG
ApaLI 101 GTGCAC G^TGCAC
AspI 1150 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 380 CCCGGG C^[CT]CG[GA]G
BbeI 891 GGCGCC GGCGC^C
BbsI 537 GAAGAC GAAGAC(2/6)
BbvCI 859 CCTCAGC CCTCAGC(-5/-2)
BciVI 463 GGATAC GTATCC(6/5)
BclI 1016 TGATCA T^GATCA
BfrI 733 CTTAAG C^TTAAG
BglII 716 AGATCT A^GATCT
BpuAI 537 GAAGAC GAAGAC(2/6)
BsaBI 1176 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 891 GGCGCC G[GA]^CG[CT]C
BsmFI 839 GGGAC GGGAC(10/14)
BsoBI 380 CCCGGG C^[CT]CG[GA]G
BspDI 1048 ATCGAT AT^CGAT
BspHI 530 TCATGA T^CATGA
BspMI 941 ACCTGC ACCTGC(4/8)
BsrFI 1255 GCCGGC [GA]^CCGG[CT]
BstAPI 936 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 320 CGCG CG^CG
BstXI 1430 CCATCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 76 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1255 GCCGGC [GA]^CCGG[CT]
ClaI 1048 ATCGAT AT^CGAT
DrdI 904 GACGAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 896 CCATGG C^C[GA][CT]GG
Eco81I 76 CCTGAGG CC^T[ACGT]AGG
EcoNI 1119 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 994 GATATC GAT^ATC
FbaI 1016 TGATCA T^GATCA
Hin1I 891 GGCGCC G[GA]^CG[CT]C
KasI 891 GGCGCC G^GCGCC
MamI 1176 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 734 TTAA T^TAA
MvnI 320 CGCG CG^CG
NaeI 1255 GCCGGC GCC^GGC
NarI 891 GGCGCC GG^CGCC
NcoI 896 CCATGG C^CATGG
NgoMIV 1255 GCCGGC G^CCGGC
NheI 1290 GCTAGC G^CTAGC
PflFI 1150 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 781 GAGTC GAGTC(4/5)
RcaI 530 TCATGA T^CATGA
SacI 337 GAGCTC GAGCT^C
SfoI 891 GGCGCC GGC^GCC
SmaI 380 CCCGGG CCC^GGG
SmlI 733 CTTAAG C^T[CT][GA]AG
TaqI 1049 TCGA T^CGA
Tru9I 734 TTAA T^TAA
Tth111I 1150 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1049 TCGA T^CGA
XcmI 665 CCAAGTCTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 380 CCCGGG C^CCGGG
XmaI 380 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 1464 GATCC GGATC(4/5)
135 GGATC
Aor51HI 115 AGCGCT AGC^GCT
445 AGCGCT
AspHI 101 GTGCAC G[AT]GC[AT]^C
337 GAGCTC
BalI 1058 TGGCCA TGG^CCA
1112 TGGCCA
BanI 522 GGTGCC G^G[CT][GA]CC
891 GGCGCC
BanII 337 GAGCTC G[GA]GC[CT]^C
921 GGGCTC
BbrPI 241 CACGTG CAC^GTG
267 CACGTG
BfaI 1144 CTAG C^TAG
1291 CTAG
BsaAI 241 CACGTG [CT]AC^GT[GA]
267 CACGTG
BsaI 284 GAGACC GGTCTC(1/5)
469 GGTCTC
BsiHKAI 101 GTGCAC G[AT]GC[AT]^C
337 GAGCTC
Eco47III 115 AGCGCT AGC^GCT
445 AGCGCT
EcoT14I 896 CCATGG C^C[AT][AT]GG
1092 CCAAGG
MaeI 1144 CTAG C^TAG
1291 CTAG
MaeII 242 ACGT A^CGT
268 ACGT
MluNI 1058 TGGCCA TGG^CCA
1112 TGGCCA
MscI 1058 TGGCCA TGG^CCA
1112 TGGCCA
MspA1I 181 CAGCTG C[AC]G^C[GT]G
370 CAGCTG
PflMI 366 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1401 CCAGAACCTGG
PmaCI 241 CACGTG CAC^GTG
267 CACGTG
PmlI 241 CACGTG CAC^GTG
267 CACGTG
PpuMI 839 GGGACCT [GA]G^G[AT]CC[CT]
938 AGGACCT
PvuII 181 CAGCTG CAG^CTG
370 CAGCTG
SbfI 129 CCTGCAGG CCTGCA^GG
942 CCTGCAGG
Sse8387I 129 CCTGCAGG CCTGCA^GG
942 CCTGCAGG
StyI 896 CCATGG C^C[AT][AT]GG
1092 CCAAGG
Tsp509I 304 AATT ^AATT
1042 AATT
Van91I 366 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1401 CCAGAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI TseI Tsp45I TspRI
XhoII