Restriction map for
pF1KSDA0063
##################################
# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 561 GGTACC G^GTACC
AfaI 562 GTAC GT^AC
AlwI 419 GGATC GGATC(4/5)
Asp718I 561 GGTACC G^GTACC
AspHI 502 GAGCTC G[AT]GC[AT]^C
BanI 561 GGTACC G^G[CT][GA]CC
BanII 502 GAGCTC G[GA]GC[CT]^C
BbsI 130 GTCTTC GAAGAC(2/6)
BcgI 311 GCAGGGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 159 GGATAC GTATCC(6/5)
BpmI 369 CTCCAG CTGGAG(16/14)
BpuAI 130 GTCTTC GAAGAC(2/6)
BsiHKAI 502 GAGCTC G[AT]GC[AT]^C
BsmFI 302 GGGAC GGGAC(10/14)
BspHI 356 TCATGA T^CATGA
BsrI 416 ACTGG ACTGG(1/-1)
BstF5I 315 GGATG GGATG(2/0)
BstYI 419 GGATCT [GA]^GATC[CT]
DdeI 505 CTCAG C^T[ACGT]AG
DpnII 420 GATC ^GATC
DraII 442 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 442 GGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 315 GGATG GGATG(9/13)
HapII 156 CCGG C^CGG
HpaII 156 CCGG C^CGG
KpnI 561 GGTACC GGTAC^C
MaeIII 205 GTGAC ^GT[ACGT]AC
MboI 420 GATC ^GATC
MflI 419 GGATCT [GA]^GATC[CT]
MslI 198 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 156 CCGG C^CGG
NdeII 420 GATC ^GATC
NspI 224 GCATGC [GA]CATG^[CT]
PleI 460 GACTC GAGTC(4/5)
RcaI 356 TCATGA T^CATGA
RsaI 562 GTAC GT^AC
SacI 502 GAGCTC GAGCT^C
SapI 219 GAAGAGC GCTCTTC(1/4)
Sau3AI 420 GATC ^GATC
SfaNI 474 GATGC GCATC(5/9)
SmaI 155 CCCGGG CCC^GGG
SmlI 469 CTCAAG C^T[CT][GA]AG
SphI 224 GCATGC GCATG^C
Tsp45I 205 GTGAC ^GT[GC]AC
XhoII 419 GGATCT [GA]^GATC[CT]
XmaCI 155 CCCGGG C^CCGGG
XmaI 155 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AvaI 155 CCCGGG C^[CT]CG[GA]G
478 CCCGAG
AvaII 385 GGTCC G^G[AT]CC
596 GGACC
BcnI 155 CCCGG CC^[GC]GG
156 CCGGG
BmyI 107 GTGCCC G[AGT]GC[ACT]^C
502 GAGCTC
BsmAI 23 GAGAC GTCTC(1/5)
189 GTCTC
BsoBI 155 CCCGGG C^[CT]CG[GA]G
478 CCCGAG
Bsp1286I 107 GTGCCC G[AGT]GC[ACT]^C
502 GAGCTC
BsrDI 327 CATTGC GCAATG(2/0)
143 GCAATG
BstNI 57 CCAGG CC^[AT]GG
135 CCAGG
Cac8I 224 GCATGC GC[ACGT]^[ACGT]GC
500 GCGAGC
CfoI 93 GCGC GCG^C
309 GCGC
DsaI 13 CCATGG C^C[GA][CT]GG
200 CCATGG
EarI 219 GAAGAG CTCTTC(1/4)
568 GAAGAG
EcoRII 57 CCAGG ^CC[AT]GG
135 CCAGG
EcoT14I 13 CCATGG C^C[AT][AT]GG
200 CCATGG
HaeIII 30 GGCC GG^CC
444 GGCC
HhaI 93 GCGC GCG^C
309 GCGC
HinP1I 93 GCGC G^CGC
309 GCGC
HphI 204 GGTGA GGTGA(8/7)
152 TCACC
Ksp632I 219 GAAGAG CTCTTC(1/4)
568 GAAGAG
MaeII 128 ACGT A^CGT
341 ACGT
MvaI 57 CCAGG CC^[AT]GG
135 CCAGG
MwoI 93 GCGCAGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
109 GCCCTCCACGC
NciI 155 CCCGG CC^[GC]GG
156 CCGGG
NcoI 13 CCATGG C^CATGG
200 CCATGG
StyI 13 CCATGG C^C[AT][AT]GG
200 CCATGG
TaqI 429 TCGA T^CGA
458 TCGA
TfiI 40 GAATC G^A[AT]TC
360 GAATC
TspRI 389 CACTG CA[GC]TG[ACGT][ACGT]^
415 CACTG
TthHB8I 429 TCGA T^CGA
458 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BssKI Cfr13I Fnu4HI HinfI ItaI
MboII MnlI NlaIII NlaIV Sau96I
ScrFI TseI