################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I FauI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Alw44I 1045 GTGCAC G^TGCAC AlwNI 79 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1045 GTGCAC G^TGCAC AvaII 87 GGACC G^G[AT]CC BbsI 2303 GAAGAC GAAGAC(2/6) BclI 261 TGATCA T^GATCA BfaI 92 CTAG C^TAG BpmI 124 CTGGAG CTGGAG(16/14) BpuAI 2303 GAAGAC GAAGAC(2/6) BsrI 247 CCAGT ACTGG(1/-1) Cfr13I 87 GGACC G^G[ACGT]CC DraI 40 TTTAAA TTT^AAA DraIII 2390 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 208 CCATGG C^C[GA][CT]GG EcoNI 681 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 795 GATATC GAT^ATC EcoT14I 208 CCATGG C^C[AT][AT]GG EcoT22I 1496 ATGCAT ATGCA^T FbaI 261 TGATCA T^GATCA HincII 1692 GTCAAC GT[CT]^[GA]AC HindII 1692 GTCAAC GT[CT]^[GA]AC MaeI 92 CTAG C^TAG MspA1I 257 CAGCTG C[AC]G^C[GT]G NcoI 208 CCATGG C^CATGG NsiI 1496 ATGCAT ATGCA^T PshAI 88 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 257 CAGCTG CAG^CTG SapI 202 GAAGAGC GCTCTTC(1/4) Sau96I 87 GGACC G^G[ACGT]CC SfcI 132 CTACAG C^T[GA][CT]AG SspI 532 AATATT AAT^ATT StyI 208 CCATGG C^C[AT][AT]GG TaqI 1032 TCGA T^CGA TthHB8I 1032 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AspHI 643 GAGCAC G[AT]GC[AT]^C 1045 GTGCAC BanII 101 GGGCTC G[GA]GC[CT]^C 2135 GGGCTC BsiHKAI 643 GAGCAC G[AT]GC[AT]^C 1045 GTGCAC BsmAI 610 GAGAC GTCTC(1/5) 145 GTCTC BsmI 1153 GAATGC GAATGC(1/-1) 2077 GAATGC Bsp1407I 1956 TGTACA T^GTACA 2292 TGTACA BspHI 603 TCATGA T^CATGA 1746 TCATGA BsrDI 1157 GCAATG GCAATG(2/0) 2081 GCAATG BsrGI 1956 TGTACA T^GTACA 2292 TGTACA MslI 1384 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1747 CATGAATGTG NlaIV 87 GGACCC GG[ACGT]^[ACGT]CC 278 GGAGCC PleI 28 GAGTC GAGTC(4/5) 597 GAGTC RcaI 603 TCATGA T^CATGA 1746 TCATGA SmlI 557 CTCAAG C^T[CT][GA]AG 1411 CTTGAG SspBI 1956 TGTACA T^GTACA 2292 TGTACA TfiI 424 GATTC G^A[AT]TC 630 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I BbvI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI DdeI DpnII EarI EcoRI EcoRII Fnu4HI FokI HindIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NspI RsaI Sau3AI ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI XmnI