Restriction map for
pF1KSDA0067
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccII AccIII AclI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BcgI
BclI BglI BlnI BlpI Bpu1102I
BseAI BsiWI BsmBI BspEI BspHI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I CelII CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI FbaI FseI FspI HaeII
KasI KpnI KspI MfeI MluI
MroI MslI MunI MvnI NarI
NotI NruI NspV PacI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 3863 GACGTC GACGT^C
AflII 468 CTTAAG C^TTAAG
Alw44I 2373 GTGCAC G^TGCAC
AocI 3077 CCTCAGG CC^T[ACGT]AGG
ApaI 1670 GGGCCC GGGCC^C
ApaLI 2373 GTGCAC G^TGCAC
AspI 3300 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1050 TGGCCA TGG^CCA
BanI 646 GGCACC G^G[CT][GA]CC
BbrPI 1080 CACGTG CAC^GTG
BfrI 468 CTTAAG C^TTAAG
BglII 2850 AGATCT A^GATCT
BsaAI 1080 CACGTG [CT]AC^GT[GA]
BsaBI 808 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1894 GGTCTC GGTCTC(1/5)
BsiEI 3138 CGACCG CG[GA][CT]^CG
BsmI 3853 GAATGC GAATGC(1/-1)
Bsp1407I 159 TGTACA T^GTACA
BspDI 130 ATCGAT AT^CGAT
BspLU11I 2369 ACATGT A^CATGT
BsrBI 1666 GAGCGG CCGCTC(-3/-3)
BsrDI 2466 CATTGC GCAATG(2/0)
BsrGI 159 TGTACA T^GTACA
BstEII 586 GGTGACC G^GT[ACGT]ACC
BstPI 586 GGTGACC G^GT[ACGT]ACC
Bsu36I 3077 CCTCAGG CC^T[ACGT]AGG
CfoI 3569 GCGC GCG^C
ClaI 130 ATCGAT AT^CGAT
DrdI 589 GACCTGATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3077 CCTCAGG CC^T[ACGT]AGG
EcoO65I 586 GGTGACC G^GT[ACGT]ACC
EcoRI 608 GAATTC G^AATTC
EcoRV 3394 GATATC GAT^ATC
FauI 1668 GCGGG CCCGC(4/6)
HhaI 3569 GCGC GCG^C
HinP1I 3569 GCGC G^CGC
HpaI 1849 GTTAAC GTT^AAC
MamI 808 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1050 TGGCCA TGG^CCA
MscI 1050 TGGCCA TGG^CCA
NaeI 941 GCCGGC GCC^GGC
NdeI 1985 CATATG CA^TATG
NgoMIV 941 GCCGGC G^CCGGC
NheI 180 GCTAGC G^CTAGC
PaeR7I 1751 CTCGAG C^TCGAG
PflFI 3300 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2848 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1080 CACGTG CAC^GTG
PmlI 1080 CACGTG CAC^GTG
PpuMI 2687 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1731 CTGCAG CTGCA^G
SapI 519 GAAGAGC GCTCTTC(1/4)
ScaI 2555 AGTACT AGT^ACT
SpeI 3238 ACTAGT A^CTAGT
SspBI 159 TGTACA T^GTACA
StuI 3068 AGGCCT AGG^CCT
Tth111I 3300 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2848 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1751 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 1841 GGCGTC G[GA]^CG[CT]C
3863 GACGTC
AflIII 1252 ACACGT A^C[GA][CT]GT
2369 ACATGT
AgeI 1315 ACCGGT A^CCGGT
2381 ACCGGT
BamHI 1127 GGATCC G^GATCC
1980 GGATCC
BbvCI 1211 CCTCAGC CCTCAGC(-5/-2)
1616 CCTCAGC
BsaHI 1841 GGCGTC G[GA]^CG[CT]C
3863 GACGTC
BsaWI 1315 ACCGGT [AT]^CCGG[AT]
2381 ACCGGT
BsgI 1220 CTGCAC GTGCAG(16/14)
1526 CTGCAC
BstXI 663 CCAGACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2661 CCAGGAAGATGG
EaeI 943 CGGCCA [CT]^GGCC[GA]
1050 TGGCCA
EcoT22I 251 ATGCAT ATGCA^T
3244 ATGCAT
HgaI 1842 GCGTC GACGC(5/10)
3468 GCGTC
Hin1I 1841 GGCGTC G[GA]^CG[CT]C
3863 GACGTC
HindIII 1708 AAGCTT A^AGCTT
3637 AAGCTT
NsiI 251 ATGCAT ATGCA^T
3244 ATGCAT
PinAI 1315 ACCGGT A^CCGGT
2381 ACCGGT
SmaI 2136 CCCGGG CCC^GGG
2792 CCCGGG
SphI 602 GCATGC GCATG^C
1658 GCATGC
XmaCI 2136 CCCGGG C^CCGGG
2792 CCCGGG
XmaI 2136 CCCGGG C^CCGGG
2792 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI