Restriction map for
pF1KSDA0074
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AviII AvrII BalI BamHI
BbrPI BbvCI BciVI BclI BfaI
BglI BlnI BsaAI BsaBI BsaI
BsiEI BsiHKAI BsiWI Bsp1407I BspDI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
Bsu36I Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MscI MunI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 1975 TCCGGA T^CCGGA
AcsI 1895 AAATTC [GA]^AATT[CT]
AcyI 123 GGCGCC G[GA]^CG[CT]C
AflII 1591 CTTAAG C^TTAAG
AlwNI 1170 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1968 AGCGCT AGC^GCT
ApoI 1895 AAATTC [GA]^AATT[CT]
Asp700I 157 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 123 GGCGCC GGCGC^C
BfrI 1591 CTTAAG C^TTAAG
BsaHI 123 GGCGCC G[GA]^CG[CT]C
BsaWI 1975 TCCGGA [AT]^CCGG[AT]
BseAI 1975 TCCGGA T^CCGGA
BsmBI 52 GAGACG CGTCTC(1/5)
BsmI 378 GAATGC GAATGC(1/-1)
BspEI 1975 TCCGGA T^CCGGA
BspHI 1158 TCATGA T^CATGA
BspMI 2078 ACCTGC ACCTGC(4/8)
BsrBI 187 GAGCGG CCGCTC(-3/-3)
Bst1107I 526 GTATAC GTA^TAC
BstZ17I 526 GTATAC GTA^TAC
DrdI 1549 GACACTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 851 CCACGG C^C[GA][CT]GG
EaeI 956 TGGCCG [CT]^GGCC[GA]
Eco47III 1968 AGCGCT AGC^GCT
EcoNI 263 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 123 GGCGCC G[GA]^CG[CT]C
KasI 123 GGCGCC G^GCGCC
MroI 1975 TCCGGA T^CCGGA
MspA1I 196 CAGCGG C[AC]G^C[GT]G
NarI 123 GGCGCC GG^CGCC
NdeI 1844 CATATG CA^TATG
NspI 1949 GCATGT [GA]CATG^[CT]
RcaI 1158 TCATGA T^CATGA
SfoI 123 GGCGCC GGC^GCC
StuI 1342 AGGCCT AGG^CCT
XmnI 157 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 526 GTATAC GT^[AC][GT]AC
1018 GTAGAC
AccII 56 CGCG CG^CG
502 CGCG
AfaI 467 GTAC GT^AC
1394 GTAC
AspI 1302 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1479 GACCAAGTC
AvaI 207 CTCGAG C^[CT]CG[GA]G
865 CTCGAG
BanII 1183 GAGCCC G[GA]GC[CT]^C
2065 GGGCTC
BcgI 104 CGATGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2072 CGAAGGACCTGC
BglII 488 AGATCT A^GATCT
941 AGATCT
BlpI 1867 GCTGAGC GC^T[ACGT]AGC
2058 GCTTAGC
Bpu1102I 1867 GCTGAGC GC^T[ACGT]AGC
2058 GCTTAGC
BseRI 78 CTCCTC GAGGAG(10/8)
2085 GAGGAG
BsgI 762 GTGCAG GTGCAG(16/14)
926 GTGCAG
BsoBI 207 CTCGAG C^[CT]CG[GA]G
865 CTCGAG
BstUI 56 CGCG CG^CG
502 CGCG
BstXI 1916 CCAAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2120 CCATACCTCTGG
BstYI 488 AGATCT [GA]^GATC[CT]
941 AGATCT
CelII 1867 GCTGAGC GC^T[ACGT]AGC
2058 GCTTAGC
DraI 451 TTTAAA TTT^AAA
903 TTTAAA
FauI 463 GCGGG CCCGC(4/6)
2063 GCGGG
HgaI 54 GACGC GACGC(5/10)
1349 GACGC
HincII 1066 GTTGAC GT[CT]^[GA]AC
1546 GTTGAC
HindII 1066 GTTGAC GT[CT]^[GA]AC
1546 GTTGAC
MflI 488 AGATCT [GA]^GATC[CT]
941 AGATCT
MslI 517 CATGCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1305 CATGTCGATG
MvnI 56 CGCG CG^CG
502 CGCG
PaeR7I 207 CTCGAG C^TCGAG
865 CTCGAG
PflFI 1302 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1479 GACCAAGTC
RsaI 467 GTAC GT^AC
1394 GTAC
SexAI 1040 ACCAGGT A^CC[AT]GGT
1582 ACCAGGT
SspI 133 AATATT AAT^ATT
1280 AATATT
TfiI 417 GATTC G^A[AT]TC
2166 GAATC
Tsp45I 1076 GTGAC ^GT[GC]AC
1808 GTGAC
Tth111I 1302 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1479 GACCAAGTC
XhoI 207 CTCGAG C^TCGAG
865 CTCGAG
XhoII 488 AGATCT [GA]^GATC[CT]
941 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XcmI