Restriction map for
pF1KSDA0076
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BamHI BbeI
BfrI BsiWI Bsp1407I BspDI BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KpnI MfeI MluI
MunI NarI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 4858 GACGTC GACGT^C
AccIII 2025 TCCGGA T^CCGGA
AflIII 503 ACATGT A^C[GA][CT]GT
Alw44I 1267 GTGCAC G^TGCAC
Aor51HI 2849 AGCGCT AGC^GCT
ApaLI 1267 GTGCAC G^TGCAC
AvrII 2898 CCTAGG C^CTAGG
BciVI 4002 GGATAC GTATCC(6/5)
BclI 1676 TGATCA T^GATCA
BlnI 2898 CCTAGG C^CTAGG
BsaBI 97 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2025 TCCGGA [AT]^CCGG[AT]
BseAI 2025 TCCGGA T^CCGGA
BsiEI 4914 CGACCG CG[GA][CT]^CG
BspEI 2025 TCCGGA T^CCGGA
BspHI 3416 TCATGA T^CATGA
BspLU11I 503 ACATGT A^CATGT
CpoI 3818 CGGACCG CG^G[AT]CCG
Eco47III 2849 AGCGCT AGC^GCT
EcoRI 25 GAATTC G^AATTC
FbaI 1676 TGATCA T^GATCA
KspI 3252 CCGCGG CCGC^GG
MamI 97 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2025 TCCGGA T^CCGGA
MseI 3527 TTAA T^TAA
NheI 663 GCTAGC G^CTAGC
RcaI 3416 TCATGA T^CATGA
RsrII 3818 CGGACCG CG^G[AT]CCG
SacII 3252 CCGCGG CCGC^GG
SapI 747 GCTCTTC GCTCTTC(1/4)
SspI 3529 AATATT AAT^ATT
Tru9I 3527 TTAA T^TAA
XcmI 1352 CCAGTAGAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccI 1690 GTCTAC GT^[AC][GT]AC
3958 GTCTAC
AcsI 25 GAATTC [GA]^AATT[CT]
3066 GAATTT
AcyI 4858 GACGTC G[GA]^CG[CT]C
4904 GACGCC
ApoI 25 GAATTC [GA]^AATT[CT]
3066 GAATTT
BbrPI 424 CACGTG CAC^GTG
3643 CACGTG
BglII 218 AGATCT A^GATCT
1286 AGATCT
BsaAI 424 CACGTG [CT]AC^GT[GA]
3643 CACGTG
BsaHI 4858 GACGTC G[GA]^CG[CT]C
4904 GACGCC
BsaI 3837 GGTCTC GGTCTC(1/5)
4443 GGTCTC
BsmI 716 GCATTC GAATGC(1/-1)
2879 GCATTC
BsrDI 3738 GCAATG GCAATG(2/0)
4153 GCAATG
BssSI 2113 CACGAG CACGAG(-5/-1)
4543 CACGAG
DrdI 475 GACCCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4756 GACCAGCTTGTC
Hin1I 4858 GACGTC G[GA]^CG[CT]C
4904 GACGCC
HincII 869 GTCAAC GT[CT]^[GA]AC
1245 GTCAAC
HindII 869 GTCAAC GT[CT]^[GA]AC
1245 GTCAAC
NaeI 2611 GCCGGC GCC^GGC
2703 GCCGGC
NcoI 896 CCATGG C^CATGG
4731 CCATGG
NgoMIV 2611 GCCGGC G^CCGGC
2703 GCCGGC
PaeR7I 2691 CTCGAG C^TCGAG
3840 CTCGAG
PmaCI 424 CACGTG CAC^GTG
3643 CACGTG
PmlI 424 CACGTG CAC^GTG
3643 CACGTG
SacI 963 GAGCTC GAGCT^C
3381 GAGCTC
SmaI 3395 CCCGGG CCC^GGG
5039 CCCGGG
SphI 2343 GCATGC GCATG^C
4843 GCATGC
StuI 944 AGGCCT AGG^CCT
2981 AGGCCT
XhoI 2691 CTCGAG C^TCGAG
3840 CTCGAG
XmaCI 3395 CCCGGG C^CCGGG
5039 CCCGGG
XmaI 3395 CCCGGG C^CCGGG
5039 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII