Restriction map for
pF1KSDA0098
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BcnI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsgI
BsiEI BsiWI BsoBI BspDI BspHI
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FseI FspI HindIII HpaI KpnI
KspI MluI MluNI MscI MspA1I
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 1226 TCCGGA T^CCGGA
AcsI 850 AAATTT [GA]^AATT[CT]
AfaI 1075 GTAC GT^AC
AflII 231 CTTAAG C^TTAAG
AflIII 754 ACATGT A^C[GA][CT]GT
AhdI 67 GACCGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 850 AAATTT [GA]^AATT[CT]
AspEI 67 GACCGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1039 GAGCTC G[AT]GC[AT]^C
BbeI 38 GGCGCC GGCGC^C
BcgI 414 GCAGGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 231 CTTAAG C^TTAAG
BglII 1082 AGATCT A^GATCT
BsaWI 1226 TCCGGA [AT]^CCGG[AT]
BseAI 1226 TCCGGA T^CCGGA
BsiHKAI 1039 GAGCTC G[AT]GC[AT]^C
BsmBI 605 GAGACG CGTCTC(1/5)
Bsp1407I 1074 TGTACA T^GTACA
BspEI 1226 TCCGGA T^CCGGA
BspLU11I 754 ACATGT A^CATGT
BsrBI 598 GAGCGG CCGCTC(-3/-3)
BsrGI 1074 TGTACA T^GTACA
BstYI 1082 AGATCT [GA]^GATC[CT]
CfoI 39 GCGC GCG^C
Eam1105I 67 GACCGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 856 GAAGAG CTCTTC(1/4)
HaeII 38 GGCGCC [GA]GCGC^[CT]
HapII 1227 CCGG C^CGG
HhaI 39 GCGC GCG^C
HinP1I 39 GCGC G^CGC
HpaII 1227 CCGG C^CGG
KasI 38 GGCGCC G^GCGCC
Ksp632I 856 GAAGAG CTCTTC(1/4)
MaeII 608 ACGT A^CGT
MfeI 364 CAATTG C^AATTG
MflI 1082 AGATCT [GA]^GATC[CT]
MroI 1226 TCCGGA T^CCGGA
MspI 1227 CCGG C^CGG
MunI 364 CAATTG C^AATTG
NarI 38 GGCGCC GG^CGCC
RsaI 1075 GTAC GT^AC
SacI 1039 GAGCTC GAGCT^C
SfoI 38 GGCGCC GGC^GCC
SspBI 1074 TGTACA T^GTACA
XcmI 263 CCAAGCTGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1082 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 424 CGCG CG^CG
1240 CGCG
AcyI 3 GGCGTC G[GA]^CG[CT]C
38 GGCGCC
BanI 38 GGCGCC G^G[CT][GA]CC
340 GGTGCC
BanII 519 GGGCTC G[GA]GC[CT]^C
1039 GAGCTC
BclI 249 TGATCA T^GATCA
1178 TGATCA
BfaI 370 CTAG C^TAG
895 CTAG
BsaBI 244 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
996 GATTGCCATC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
38 GGCGCC
BsmI 570 GAATGC GAATGC(1/-1)
380 GCATTC
BsrI 883 ACTGG ACTGG(1/-1)
1362 ACTGG
BstUI 424 CGCG CG^CG
1240 CGCG
BstXI 263 CCAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
317 CCACAGGAGTGG
DsaI 8 CCATGG C^C[GA][CT]GG
1376 CCATGG
EcoRV 1278 GATATC GAT^ATC
1548 GATATC
EcoT14I 8 CCATGG C^C[AT][AT]GG
1376 CCATGG
FbaI 249 TGATCA T^GATCA
1178 TGATCA
Hin1I 3 GGCGTC G[GA]^CG[CT]C
38 GGCGCC
MaeI 370 CTAG C^TAG
895 CTAG
MamI 244 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
996 GATTGCCATC
MslI 318 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1371 CATCCCCATG
MvnI 424 CGCG CG^CG
1240 CGCG
NcoI 8 CCATGG C^CATGG
1376 CCATGG
NspI 754 ACATGT [GA]CATG^[CT]
1512 GCATGT
PpuMI 13 GGGACCC [GA]G^G[AT]CC[CT]
978 AGGACCT
PshAI 804 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1422 GACCGAAGTC
SfcI 128 CTGTAG C^T[GA][CT]AG
584 CTGTAG
StyI 8 CCATGG C^C[AT][AT]GG
1376 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HgaI HincII HindII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I