Restriction map for
pF1KA0102
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AcsI 228 GAATTT [GA]^AATT[CT]
ApoI 228 GAATTT [GA]^AATT[CT]
BamHI 453 GGATCC G^GATCC
BcgI 413 GCATCTTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 480 CTCCAG CTGGAG(16/14)
BsiYI 71 CCAACTGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 71 CCAACTGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI 576 CATTGC GCAATG(2/0)
BssKI 638 CCAGG ^CC[ACGT]GG
BssSI 421 CTCGTG CACGAG(-5/-1)
BstNI 638 CCAGG CC^[AT]GG
Cfr13I 426 GGCCC G^G[ACGT]CC
DdeI 581 CTAAG C^T[ACGT]AG
EaeI 90 TGGCCG [CT]^GGCC[GA]
EarI 408 GAAGAG CTCTTC(1/4)
EcoRII 638 CCAGG ^CC[AT]GG
EcoT22I 617 ATGCAT ATGCA^T
HphI 254 TCACC GGTGA(8/7)
Ksp632I 408 GAAGAG CTCTTC(1/4)
MseI 488 TTAA T^TAA
MvaI 638 CCAGG CC^[AT]GG
NdeI 620 CATATG CA^TATG
NsiI 617 ATGCAT ATGCA^T
PleI 325 GAGTC GAGTC(4/5)
PshAI 603 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 408 GAAGAGC GCTCTTC(1/4)
Sau96I 426 GGCCC G^G[ACGT]CC
ScrFI 638 CCAGG CC^[ACGT]GG
Tru9I 488 TTAA T^TAA
XcmI 329 CCAAACCCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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BglI 92 GCCGTAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
283 GCCATAGTGGC
BsmFI 79 GGGAC GGGAC(10/14)
601 GGGAC
Bsp1407I 15 TGTACA T^GTACA
262 TGTACA
BsrGI 15 TGTACA T^GTACA
262 TGTACA
BsrI 606 ACTGG ACTGG(1/-1)
482 CCAGT
BstYI 438 AGATCC [GA]^GATC[CT]
453 GGATCC
HaeIII 91 GGCC GG^CC
426 GGCC
MboII 117 GAAGA GAAGA(8/7)
408 GAAGA
MflI 438 AGATCC [GA]^GATC[CT]
453 GGATCC
NlaIII 612 CATG CATG^
646 CATG
NlaIV 453 GGATCC GG[ACGT]^[ACGT]CC
641 GGCTCC
SfaNI 616 GATGC GCATC(5/9)
413 GCATC
SfcI 48 CTGTAG C^T[GA][CT]AG
443 CTACAG
SspBI 15 TGTACA T^GTACA
262 TGTACA
TfiI 184 GATTC G^A[AT]TC
378 GATTC
XhoII 438 AGATCC [GA]^GATC[CT]
453 GGATCC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BstF5I DpnII FauI Fnu4HI FokI
HinfI ItaI MboI MnlI MspA1I
MwoI NdeII PvuII RsaI Sau3AI
TseI Tsp509I TspRI