Restriction map for
pF1KSDA0108
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AgeI 35 ACCGGT A^CCGGT
AlwI 423 GGATC GGATC(4/5)
AspI 355 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 98 GGACC G^G[AT]CC
BpmI 471 CTCCAG CTGGAG(16/14)
BsaWI 35 ACCGGT [AT]^CCGG[AT]
BseRI 620 CTCCTC GAGGAG(10/8)
BsmAI 256 GTCTC GTCTC(1/5)
BsmBI 255 CGTCTC CGTCTC(1/5)
BspMI 693 ACCTGC ACCTGC(4/8)
BsrFI 35 ACCGGT [GA]^CCGG[CT]
BstAPI 47 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 171 GCCAGC GC[ACGT]^[ACGT]GC
Cfr10I 35 ACCGGT [GA]^CCGG[CT]
Cfr13I 98 GGACC G^G[ACGT]CC
DraII 97 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 48 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 650 TGGCCG [CT]^GGCC[GA]
EcoO109I 97 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 464 CCTTGG C^C[AT][AT]GG
HincII 178 GTCAAC GT[CT]^[GA]AC
HindII 178 GTCAAC GT[CT]^[GA]AC
HphI 392 TCACC GGTGA(8/7)
MboII 335 TCTTC GAAGA(8/7)
PflFI 355 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 35 ACCGGT A^CCGGT
PpuMI 97 GGGACCT [GA]G^G[AT]CC[CT]
Sau96I 98 GGACC G^G[ACGT]CC
SexAI 100 ACCTGGT A^CC[AT]GGT
SfcI 42 CTACAG C^T[GA][CT]AG
StyI 464 CCTTGG C^C[AT][AT]GG
TspRI 30 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 355 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AluI 174 AGCT AG^CT
475 AGCT
BbvI 56 GCTGC GCAGC(8/12)
476 GCTGC
BcnI 50 CCCGG CC^[GC]GG
80 CCGGG
BsaJI 93 CCTGGG C^C[ACGT][ACGT]GG
464 CCTTGG
BsiYI 120 CCTATTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
459 CCTGGCCTTGG
BslI 120 CCTATTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
459 CCTGGCCTTGG
BsmFI 82 GGGAC GGGAC(10/14)
97 GGGAC
BstF5I 90 CATCC GGATG(2/0)
157 CATCC
DpnII 87 GATC ^GATC
424 GATC
FokI 90 CATCC GGATG(9/13)
157 CATCC
HaeIII 462 GGCC GG^CC
651 GGCC
MaeII 581 ACGT A^CGT
629 ACGT
MboI 87 GATC ^GATC
424 GATC
MseI 524 TTAA T^TAA
539 TTAA
MspA1I 173 CAGCTG C[AC]G^C[GT]G
474 CAGCTG
NciI 50 CCCGG CC^[GC]GG
80 CCGGG
NdeII 87 GATC ^GATC
424 GATC
NlaIV 23 GGAACC GG[ACGT]^[ACGT]CC
97 GGGACC
PleI 153 GACTC GAGTC(4/5)
469 GACTC
PvuII 173 CAGCTG CAG^CTG
474 CAGCTG
Sau3AI 87 GATC ^GATC
424 GATC
TfiI 327 GATTC G^A[AT]TC
407 GAATC
Tru9I 524 TTAA T^TAA
539 TTAA
TseI 56 GCTGC G^C[AT]GC
476 GCTGC
Tsp45I 32 GTGAC ^GT[GC]AC
151 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AfaI BssKI BstNI DdeI
EcoRII Fnu4HI HapII HinfI HpaII
ItaI MaeIII MnlI MspI MvaI
MwoI NlaIII RsaI ScrFI Tsp509I