Restriction map for
pF1KSDA0115
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MflI MluI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 191 TCCGGA T^CCGGA
AcsI 271 GAATTC [GA]^AATT[CT]
AcyI 828 GGCGCC G[GA]^CG[CT]C
AflIII 1148 ACGTGT A^C[GA][CT]GT
AlwNI 20 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1256 AGCGCT AGC^GCT
ApaI 80 GGGCCC GGGCC^C
ApoI 271 GAATTC [GA]^AATT[CT]
AspHI 217 GAGCTC G[AT]GC[AT]^C
AvaI 71 CCCGGG C^[CT]CG[GA]G
BalI 501 TGGCCA TGG^CCA
BanI 828 GGCGCC G^G[CT][GA]CC
BbeI 828 GGCGCC GGCGC^C
BbsI 1333 GTCTTC GAAGAC(2/6)
BpuAI 1333 GTCTTC GAAGAC(2/6)
BsaHI 828 GGCGCC G[GA]^CG[CT]C
BsaI 340 GAGACC GGTCTC(1/5)
BseAI 191 TCCGGA T^CCGGA
BsiHKAI 217 GAGCTC G[AT]GC[AT]^C
BsoBI 71 CCCGGG C^[CT]CG[GA]G
BspEI 191 TCCGGA T^CCGGA
BsrDI 729 CATTGC GCAATG(2/0)
BsrFI 1184 ACCGGC [GA]^CCGG[CT]
BstAPI 425 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 403 GGTGACC G^GT[ACGT]ACC
BstPI 403 GGTGACC G^GT[ACGT]ACC
BstXI 1301 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1184 ACCGGC [GA]^CCGG[CT]
DraI 1168 TTTAAA TTT^AAA
DsaI 65 CCGCGG C^C[GA][CT]GG
Eco47III 1256 AGCGCT AGC^GCT
EcoO65I 403 GGTGACC G^GT[ACGT]ACC
EcoRI 271 GAATTC G^AATTC
EcoT14I 498 CCTTGG C^C[AT][AT]GG
HgaI 761 GCGTC GACGC(5/10)
Hin1I 828 GGCGCC G[GA]^CG[CT]C
KasI 828 GGCGCC G^GCGCC
KspI 65 CCGCGG CCGC^GG
MluNI 501 TGGCCA TGG^CCA
MroI 191 TCCGGA T^CCGGA
MscI 501 TGGCCA TGG^CCA
MseI 1169 TTAA T^TAA
NarI 828 GGCGCC GG^CGCC
NspI 702 ACATGC [GA]CATG^[CT]
PvuII 1060 CAGCTG CAG^CTG
SacI 217 GAGCTC GAGCT^C
SacII 65 CCGCGG CCGC^GG
SfoI 828 GGCGCC GGC^GCC
SmaI 71 CCCGGG CCC^GGG
StyI 498 CCTTGG C^C[AT][AT]GG
Tru9I 1169 TTAA T^TAA
Tsp509I 272 AATT ^AATT
XmaCI 71 CCCGGG C^CCGGG
XmaI 71 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 6 GTAC GT^AC
1206 GTAC
BciVI 696 GTATCC GTATCC(6/5)
1222 GTATCC
BsaWI 11 TCCGGT [AT]^CCGG[AT]
191 TCCGGA
BsgI 17 GTGCAG GTGCAG(16/14)
817 GTGCAG
BsmFI 933 GTCCC GGGAC(10/14)
1036 GTCCC
BspMI 536 ACCTGC ACCTGC(4/8)
19 GCAGGT
BsrI 693 CCAGT ACTGG(1/-1)
1164 CCAGT
BstNI 741 CCAGG CC^[AT]GG
746 CCAGG
EcoNI 32 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
537 CCTGCTGAAGG
EcoRII 741 CCAGG ^CC[AT]GG
746 CCAGG
HinfI 174 GACTC G^A[ACGT]TC
796 GACTC
HphI 403 GGTGA GGTGA(8/7)
689 TCACC
MaeIII 404 GTGAC ^GT[ACGT]AC
979 GTCAC
MvaI 741 CCAGG CC^[AT]GG
746 CCAGG
PleI 174 GACTC GAGTC(4/5)
796 GACTC
RsaI 6 GTAC GT^AC
1206 GTAC
SapI 650 GCTCTTC GCTCTTC(1/4)
1314 GCTCTTC
Tsp45I 404 GTGAC ^GT[GC]AC
979 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MaeI MaeII MboI MboII MnlI
MslI MspA1I MspI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI TspRI TthHB8I