Restriction map for
pF1KSDA0120
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BlnI BpuAI BsaAI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KasI KpnI MaeI MfeI MluI
MroI MslI MunI NarI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tsp45I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcyI 549 GGCGTC G[GA]^CG[CT]C
AfaI 207 GTAC GT^AC
Aor51HI 302 AGCGCT AGC^GCT
AseI 196 ATTAAT AT^TAAT
AsnI 196 ATTAAT AT^TAAT
AspHI 190 GAGCTC G[AT]GC[AT]^C
BalI 2 TGGCCA TGG^CCA
BamHI 438 GGATCC G^GATCC
BanI 535 GGCACC G^G[CT][GA]CC
BcnI 33 CCGGG CC^[GC]GG
BlpI 298 GCTGAGC GC^T[ACGT]AGC
BpmI 76 CTGGAG CTGGAG(16/14)
Bpu1102I 298 GCTGAGC GC^T[ACGT]AGC
BsaHI 549 GGCGTC G[GA]^CG[CT]C
BsiHKAI 190 GAGCTC G[AT]GC[AT]^C
BsmAI 552 GTCTC GTCTC(1/5)
BsmBI 551 CGTCTC CGTCTC(1/5)
BsmFI 12 GGGAC GGGAC(10/14)
BspMI 15 ACCTGC ACCTGC(4/8)
BsrFI 128 GCCGGC [GA]^CCGG[CT]
CelII 298 GCTGAGC GC^T[ACGT]AGC
CfoI 303 GCGC GCG^C
Cfr10I 128 GCCGGC [GA]^CCGG[CT]
DraIII 105 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 427 CTCTTC CTCTTC(1/4)
Eco47III 302 AGCGCT AGC^GCT
FauI 545 GCGGG CCCGC(4/6)
FseI 127 GGCCGGCC GGCCGG^CC
HaeII 302 AGCGCT [GA]GCGC^[CT]
HhaI 303 GCGC GCG^C
Hin1I 549 GGCGTC G[GA]^CG[CT]C
HinP1I 303 GCGC G^CGC
HphI 101 TCACC GGTGA(8/7)
Ksp632I 427 CTCTTC CTCTTC(1/4)
KspI 543 CCGCGG CCGC^GG
MaeII 515 ACGT A^CGT
MaeIII 525 GTTAC ^GT[ACGT]AC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NaeI 128 GCCGGC GCC^GGC
NciI 33 CCGGG CC^[GC]GG
NcoI 251 CCATGG C^CATGG
NdeI 20 CATATG CA^TATG
NgoMIV 128 GCCGGC G^CCGGC
PflMI 333 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 12 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 196 ATTAAT AT^TAAT
RsaI 207 GTAC GT^AC
SacI 190 GAGCTC GAGCT^C
SacII 543 CCGCGG CCGC^GG
SapI 426 GCTCTTC GCTCTTC(1/4)
SmlI 166 CTCAAG C^T[CT][GA]AG
StuI 242 AGGCCT AGG^CCT
Van91I 333 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 145 CGCG CG^CG
544 CGCG
AlwNI 82 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
283 CAGTTCCTG
AvaI 211 CCCGAG C^[CT]CG[GA]G
413 CCCGAG
AvaII 13 GGACC G^G[AT]CC
342 GGACC
BanII 190 GAGCTC G[GA]GC[CT]^C
424 GGGCTC
BglII 72 AGATCT A^GATCT
275 AGATCT
BsaBI 67 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
84 GATCCTGATC
BsgI 40 GTGCAG GTGCAG(16/14)
373 GTGCAG
BsoBI 211 CCCGAG C^[CT]CG[GA]G
413 CCCGAG
BstNI 139 CCTGG CC^[AT]GG
240 CCAGG
BstUI 145 CGCG CG^CG
544 CGCG
DraII 12 GGGACCT [GA]G^G[ACGT]CC[CT]
221 AGGCCCC
DsaI 251 CCATGG C^C[GA][CT]GG
543 CCGCGG
EcoO109I 12 GGGACCT [GA]G^G[ACGT]CC[CT]
221 AGGCCCC
EcoRII 139 CCTGG ^CC[AT]GG
240 CCAGG
EcoT14I 251 CCATGG C^C[AT][AT]GG
464 CCAAGG
HapII 33 CCGG C^CGG
129 CCGG
HinfI 390 GAATC G^A[ACGT]TC
471 GAATC
HpaII 33 CCGG C^CGG
129 CCGG
MamI 67 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
84 GATCCTGATC
MseI 197 TTAA T^TAA
314 TTAA
MspI 33 CCGG C^CGG
129 CCGG
MvaI 139 CCTGG CC^[AT]GG
240 CCAGG
MvnI 145 CGCG CG^CG
544 CGCG
PvuII 296 CAGCTG CAG^CTG
496 CAGCTG
SfaNI 67 GATGC GCATC(5/9)
579 GATGC
StyI 251 CCATGG C^C[AT][AT]GG
464 CCAAGG
TfiI 390 GAATC G^A[AT]TC
471 GAATC
Tru9I 197 TTAA T^TAA
314 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AlwI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstYI Cac8I Cfr13I
DdeI DpnII Fnu4HI FokI HaeIII
HgaI ItaI MboI MboII MflI
MnlI MspA1I MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI XhoII