Restriction map for
pF1KSDA0122
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# NO CUT ENZYMES ( 91 enzymes) #
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AccIII AclI AcsI AflII AflIII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI AspI AvrII BbeI
BbrPI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BstBI
Bsu36I ClaI DraI EarI Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KasI Ksp632I
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SapI ScaI SexAI SfoI
SfuI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tth111I XbaI
XcmI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1699 GACGTC GACGT^C
Acc65I 1264 GGTACC G^GTACC
AhdI 1462 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2696 GTGCAC G^TGCAC
ApaLI 2696 GTGCAC G^TGCAC
Asp700I 693 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1264 GGTACC G^GTACC
AspEI 1462 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 911 TGCGCA TGC^GCA
BamHI 1416 GGATCC G^GATCC
BbsI 1350 GTCTTC GAAGAC(2/6)
BbvCI 396 GCTGAGG CCTCAGC(-5/-2)
BlpI 792 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 792 GCTGAGC GC^T[ACGT]AGC
BpuAI 1350 GTCTTC GAAGAC(2/6)
BspMI 1429 GCAGGT ACCTGC(4/8)
BsrDI 1215 CATTGC GCAATG(2/0)
BssSI 750 CACGAG CACGAG(-5/-1)
Bst1107I 1575 GTATAC GTA^TAC
BstXI 1952 CCAAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1575 GTATAC GTA^TAC
CelII 792 GCTGAGC GC^T[ACGT]AGC
CpoI 322 CGGACCG CG^G[AT]CCG
DrdI 14 GACGTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1862 CGGCCG C^GGCCG
Eam1105I 1462 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1862 CGGCCG C^GGCCG
Eco52I 1862 CGGCCG C^GGCCG
FspI 911 TGCGCA TGC^GCA
HaeII 2313 GGCGCT [GA]GCGC^[CT]
HincII 1294 GTCGAC GT[CT]^[GA]AC
HindII 1294 GTCGAC GT[CT]^[GA]AC
KpnI 1264 GGTACC GGTAC^C
KspI 1026 CCGCGG CCGC^GG
NruI 135 TCGCGA TCG^CGA
PaeR7I 2086 CTCGAG C^TCGAG
PpuMI 1431 AGGTCCC [GA]G^G[AT]CC[CT]
RsrII 322 CGGACCG CG^G[AT]CCG
SacI 1609 GAGCTC GAGCT^C
SacII 1026 CCGCGG CCGC^GG
SalI 1294 GTCGAC G^TCGAC
SbfI 1083 CCTGCAGG CCTGCA^GG
SfiI 1224 GGCCCAGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2368 CACCGGCG C[GA]^CCGG[CT]G
SmaI 219 CCCGGG CCC^GGG
Sse8387I 1083 CCTGCAGG CCTGCA^GG
Tsp509I 2141 AATT ^AATT
XhoI 2086 CTCGAG C^TCGAG
XmaCI 219 CCCGGG C^CCGGG
XmaI 219 CCCGGG C^CCGGG
XmnI 693 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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BcgI 688 CGAGAGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2544 GCAGCCTACTCG
BsaI 95 GAGACC GGTCTC(1/5)
1723 GAGACC
BsmBI 1701 CGTCTC CGTCTC(1/5)
293 GAGACG
BsrBI 60 CCGCTC CCGCTC(-3/-3)
2239 GAGCGG
BstEII 2718 GGTCACC G^GT[ACGT]ACC
2763 GGTGACC
BstPI 2718 GGTCACC G^GT[ACGT]ACC
2763 GGTGACC
DraIII 454 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
776 CACTGGGTG
EcoNI 858 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2167 CCTCCGCGAGG
EcoO65I 2718 GGTCACC G^GT[ACGT]ACC
2763 GGTGACC
PflMI 775 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2600 CCATGGGCTGG
Van91I 775 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2600 CCATGGGCTGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII