##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BcnI        BfaI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        Fnu4HI      FokI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HphI        
ItaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TseI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AflII         684  CTTAAG               C^TTAAG
AflIII        592  ACATGT               A^C[GA][CT]GT
AvaII         618  GGACC                G^G[AT]CC
BanI          368  GGCACC               G^G[CT][GA]CC
BclI          786  TGATCA               T^GATCA
BfrI          684  CTTAAG               C^TTAAG
BsaJI         372  CCAGGG               C^C[ACGT][ACGT]GG
BsaWI         469  ACCGGA               [AT]^CCGG[AT]
BsmI          545  GAATGC               GAATGC(1/-1)
BspLU11I      592  ACATGT               A^CATGT
BssKI         372  CCAGG                ^CC[ACGT]GG
BstNI         372  CCAGG                CC^[AT]GG
DpnII         787  GATC                 ^GATC
EcoRI         629  GAATTC               G^AATTC
EcoRII        372  CCAGG                ^CC[AT]GG
FbaI          786  TGATCA               T^GATCA
HaeIII        442  GGCC                 GG^CC
HapII         470  CCGG                 C^CGG
HindIII       254  AAGCTT               A^AGCTT
HpaII         470  CCGG                 C^CGG
MboI          787  GATC                 ^GATC
MspI          470  CCGG                 C^CGG
MvaI          372  CCAGG                CC^[AT]GG
NdeII         787  GATC                 ^GATC
NlaIV         368  GGCACC               GG[ACGT]^[ACGT]CC
PshAI          88  GACAATAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI        787  GATC                 ^GATC
ScaI           51  AGTACT               AGT^ACT
ScrFI         372  CCAGG                CC^[ACGT]GG
TaqI          774  TCGA                 T^CGA
TthHB8I       774  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI           18  AAATTT               [GA]^AATT[CT]
              629  GAATTC              
ApoI           18  AAATTT               [GA]^AATT[CT]
              629  GAATTC              
Asp700I       336  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              840  GAAAACCTTC          
BpmI          638  CTGGAG               CTGGAG(16/14)
              806  CTGGAG              
BseRI           3  GAGGAG               GAGGAG(10/8)
              976  GAGGAG              
BsmAI         141  GAGAC                GTCTC(1/5)
              711  GAGAC               
Cfr13I        441  GGGCC                G^G[ACGT]CC
              618  GGACC               
DdeI          158  CTGAG                C^T[ACGT]AG
              890  CTAAG               
NspI          319  ACATGC               [GA]CATG^[CT]
              592  ACATGT              
Sau96I        441  GGGCC                G^G[ACGT]CC
              618  GGACC               
Tsp509I        19  AATT                 ^AATT
              630  AATT                
XmnI          336  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              840  GAAAACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AfaI        AluI        BsiYI       BslI        BsrI        
HinfI       MaeIII      MnlI        MseI        NlaIII      
RsaI        SmlI        TfiI        Tru9I       Tsp45I      
TspRI