Restriction map for
pF1KSDA0141
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HpaI
MaeII MamI MfeI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 1163 GGTACC G^GTACC
AccII 820 CGCG CG^CG
AcsI 1175 GAATTT [GA]^AATT[CT]
AcyI 5 GGCGCC G[GA]^CG[CT]C
AlwI 303 GATCC GGATC(4/5)
ApaI 1315 GGGCCC GGGCC^C
ApoI 1175 GAATTT [GA]^AATT[CT]
Asp700I 1344 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1163 GGTACC G^GTACC
BalI 486 TGGCCA TGG^CCA
BbeI 5 GGCGCC GGCGC^C
BbsI 219 GTCTTC GAAGAC(2/6)
BlpI 505 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 505 GCTCAGC GC^T[ACGT]AGC
BpuAI 219 GTCTTC GAAGAC(2/6)
BsaHI 5 GGCGCC G[GA]^CG[CT]C
BsaI 974 GAGACC GGTCTC(1/5)
BsgI 1201 GTGCAG GTGCAG(16/14)
BspHI 858 TCATGA T^CATGA
BspMI 1160 GCAGGT ACCTGC(4/8)
BssSI 168 CACGAG CACGAG(-5/-1)
BstUI 820 CGCG CG^CG
BstXI 113 CCACTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 302 AGATCC [GA]^GATC[CT]
CelII 505 GCTCAGC GC^T[ACGT]AGC
DpnII 303 GATC ^GATC
DrdI 69 GACTCCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 5 GGCGCC [GA]GCGC^[CT]
Hin1I 5 GGCGCC G[GA]^CG[CT]C
HindIII 1061 AAGCTT A^AGCTT
KasI 5 GGCGCC G^GCGCC
KpnI 1163 GGTACC GGTAC^C
KspI 819 CCGCGG CCGC^GG
MboI 303 GATC ^GATC
MflI 302 AGATCC [GA]^GATC[CT]
MluNI 486 TGGCCA TGG^CCA
MscI 486 TGGCCA TGG^CCA
MslI 859 CATGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 820 CGCG CG^CG
NarI 5 GGCGCC GG^CGCC
NdeII 303 GATC ^GATC
NheI 1386 GCTAGC G^CTAGC
PaeR7I 1416 CTCGAG C^TCGAG
PshAI 1340 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 289 CAGCTG CAG^CTG
RcaI 858 TCATGA T^CATGA
SacII 819 CCGCGG CCGC^GG
Sau3AI 303 GATC ^GATC
SfiI 273 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5 GGCGCC GGC^GCC
SphI 594 GCATGC GCATG^C
TfiI 1209 GAATC G^A[AT]TC
Tsp509I 1176 AATT ^AATT
XcmI 979 CCAGCCTCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1416 CTCGAG C^TCGAG
XhoII 302 AGATCC [GA]^GATC[CT]
XmnI 1344 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 468 CCTCAGG CC^T[ACGT]AGG
550 CCTCAGG
BglI 274 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
437 GCCCCGATGGC
BsmAI 974 GAGAC GTCTC(1/5)
229 GTCTC
Bsu36I 468 CCTCAGG CC^T[ACGT]AGG
550 CCTCAGG
CfoI 6 GCGC GCG^C
835 GCGC
EaeI 272 TGGCCG [CT]^GGCC[GA]
486 TGGCCA
Eco81I 468 CCTCAGG CC^T[ACGT]AGG
550 CCTCAGG
EcoNI 684 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1478 CCTCCAGCAGG
HhaI 6 GCGC GCG^C
835 GCGC
HinP1I 6 GCGC G^CGC
835 GCGC
NspI 594 GCATGC [GA]CATG^[CT]
1410 ACATGC
PflMI 254 CCATATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
933 CCACAGCCTGG
SacI 29 GAGCTC GAGCT^C
542 GAGCTC
SapI 31 GCTCTTC GCTCTTC(1/4)
714 GCTCTTC
SmaI 12 CCCGGG CCC^GGG
1311 CCCGGG
StuI 1191 AGGCCT AGG^CCT
1220 AGGCCT
Tsp45I 67 GTGAC ^GT[GC]AC
697 GTGAC
Van91I 254 CCATATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
933 CCACAGCCTGG
XmaCI 12 CCCGGG C^CCGGG
1311 CCCGGG
XmaI 12 CCCGGG C^CCGGG
1311 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NcoI NlaIII NlaIV PleI PpuMI
PstI RsaI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
TspRI TthHB8I