Restriction map for
pF1KSDA0147
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# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
Alw44I ApaLI ApoI AscI Asp700I
AvrII BamHI BciVI BclI BfrI
BlnI BsaBI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI DraI
EcoNI EcoRI EcoT22I FbaI FseI
HincII HindII HindIII HpaI MamI
MfeI MluI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI Psp1406I PvuI
RcaI SalI SbfI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 3846 GGTACC G^GTACC
AccI 817 GTAGAC GT^[AC][GT]AC
AhdI 1501 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3826 AGCGCT AGC^GCT
AseI 2752 ATTAAT AT^TAAT
AsnI 2752 ATTAAT AT^TAAT
Asp718I 3846 GGTACC G^GTACC
AspEI 1501 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2737 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfaI 3896 CTAG C^TAG
BsaI 1303 GAGACC GGTCTC(1/5)
BssSI 2380 CACGAG CACGAG(-5/-1)
CpoI 1213 CGGACCG CG^G[AT]CCG
DraIII 1628 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2785 GACCACGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3974 CGGCCG C^GGCCG
Eam1105I 1501 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3974 CGGCCG C^GGCCG
Eco47III 3826 AGCGCT AGC^GCT
Eco52I 3974 CGGCCG C^GGCCG
EcoRV 277 GATATC GAT^ATC
KpnI 3846 GGTACC GGTAC^C
MaeI 3896 CTAG C^TAG
MseI 2753 TTAA T^TAA
NaeI 2407 GCCGGC GCC^GGC
NgoMIV 2407 GCCGGC G^CCGGC
NspI 1892 GCATGC [GA]CATG^[CT]
PflFI 2737 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4688 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2752 ATTAAT AT^TAAT
RsrII 1213 CGGACCG CG^G[AT]CCG
SapI 2173 GAAGAGC GCTCTTC(1/4)
ScaI 4076 AGTACT AGT^ACT
SexAI 455 ACCTGGT A^CC[AT]GGT
SphI 1892 GCATGC GCATG^C
Tru9I 2753 TTAA T^TAA
Tth111I 2737 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 3927 TCCGGA T^CCGGA
4425 TCCGGA
AfaI 3847 GTAC GT^AC
4077 GTAC
AflIII 263 ACGTGT A^C[GA][CT]GT
677 ACGTGT
AviII 182 TGCGCA TGC^GCA
380 TGCGCA
BalI 233 TGGCCA TGG^CCA
3900 TGGCCA
BglII 89 AGATCT A^GATCT
1844 AGATCT
BseAI 3927 TCCGGA T^CCGGA
4425 TCCGGA
BspEI 3927 TCCGGA T^CCGGA
4425 TCCGGA
BstEII 459 GGTGACC G^GT[ACGT]ACC
2466 GGTCACC
BstPI 459 GGTGACC G^GT[ACGT]ACC
2466 GGTCACC
EcoO65I 459 GGTGACC G^GT[ACGT]ACC
2466 GGTCACC
FspI 182 TGCGCA TGC^GCA
380 TGCGCA
KspI 3127 CCGCGG CCGC^GG
3345 CCGCGG
MluNI 233 TGGCCA TGG^CCA
3900 TGGCCA
MroI 3927 TCCGGA T^CCGGA
4425 TCCGGA
MscI 233 TGGCCA TGG^CCA
3900 TGGCCA
MslI 2412 CACTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4644 CACCCCTGTG
PaeR7I 970 CTCGAG C^TCGAG
1270 CTCGAG
RsaI 3847 GTAC GT^AC
4077 GTAC
SacII 3127 CCGCGG CCGC^GG
3345 CCGCGG
TfiI 825 GAATC G^A[AT]TC
3880 GAATC
XhoI 970 CTCGAG C^TCGAG
1270 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PleI PmaCI PmlI
PpuMI PstI PvuII SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI