Restriction map for
pF1KSDA0150
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# NO CUT ENZYMES ( 73 enzymes) #
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AatII AccI AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI BcgI BciVI BfrI BglII
BlpI Bpu1102I BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DrdI Eam1105I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 94 GGTACC G^GTACC
AcsI 442 GAATTT [GA]^AATT[CT]
Alw44I 2097 GTGCAC G^TGCAC
Aor51HI 2057 AGCGCT AGC^GCT
ApaLI 2097 GTGCAC G^TGCAC
ApoI 442 GAATTT [GA]^AATT[CT]
Asp718I 94 GGTACC G^GTACC
AspI 1514 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1895 TGCGCA TGC^GCA
AvrII 2788 CCTAGG C^CTAGG
BamHI 1911 GGATCC G^GATCC
BbeI 2368 GGCGCC GGCGC^C
BbrPI 2236 CACGTG CAC^GTG
BbvCI 1419 CCTCAGC CCTCAGC(-5/-2)
BclI 1604 TGATCA T^GATCA
BlnI 2788 CCTAGG C^CTAGG
BsiEI 277 CGGCCG CG[GA][CT]^CG
BsmBI 1347 GAGACG CGTCTC(1/5)
BsrDI 77 GCAATG GCAATG(2/0)
BstAPI 1572 GCATGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1500 GGTCACC G^GT[ACGT]ACC
BstPI 1500 GGTCACC G^GT[ACGT]ACC
BstXI 1712 CCAGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2155 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 277 CGGCCG C^GGCCG
EclXI 277 CGGCCG C^GGCCG
Eco47III 2057 AGCGCT AGC^GCT
Eco52I 277 CGGCCG C^GGCCG
EcoO65I 1500 GGTCACC G^GT[ACGT]ACC
FbaI 1604 TGATCA T^GATCA
FspI 1895 TGCGCA TGC^GCA
KasI 2368 GGCGCC G^GCGCC
KpnI 94 GGTACC GGTAC^C
KspI 2364 CCGCGG CCGC^GG
MseI 380 TTAA T^TAA
NarI 2368 GGCGCC GG^CGCC
NcoI 195 CCATGG C^CATGG
PaeR7I 519 CTCGAG C^TCGAG
PflFI 1514 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 709 CCACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2236 CACGTG CAC^GTG
PmlI 2236 CACGTG CAC^GTG
SacII 2364 CCGCGG CCGC^GG
SbfI 1915 CCTGCAGG CCTGCA^GG
ScaI 2012 AGTACT AGT^ACT
SexAI 2101 ACCAGGT A^CC[AT]GGT
SfoI 2368 GGCGCC GGC^GCC
SphI 1572 GCATGC GCATG^C
Sse8387I 1915 CCTGCAGG CCTGCA^GG
TfiI 231 GAATC G^A[AT]TC
Tru9I 380 TTAA T^TAA
Tsp509I 443 AATT ^AATT
Tth111I 1514 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 709 CCACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 519 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccIII 917 TCCGGA T^CCGGA
1343 TCCGGA
AflIII 2237 ACGTGT A^C[GA][CT]GT
2243 ACGTGT
BbsI 1473 GAAGAC GAAGAC(2/6)
1632 GAAGAC
BfaI 1235 CTAG C^TAG
2789 CTAG
BpuAI 1473 GAAGAC GAAGAC(2/6)
1632 GAAGAC
BsaAI 2236 CACGTG [CT]AC^GT[GA]
2242 TACGTG
BsaI 1297 GAGACC GGTCTC(1/5)
2469 GGTCTC
BsaWI 917 TCCGGA [AT]^CCGG[AT]
1343 TCCGGA
BseAI 917 TCCGGA T^CCGGA
1343 TCCGGA
BspEI 917 TCCGGA T^CCGGA
1343 TCCGGA
BsrBI 698 CCGCTC CCGCTC(-3/-3)
1303 CCGCTC
BssSI 2662 CACGAG CACGAG(-5/-1)
226 CTCGTG
MaeI 1235 CTAG C^TAG
2789 CTAG
MroI 917 TCCGGA T^CCGGA
1343 TCCGGA
MslI 73 CACGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
714 CACTGGCGTG
PleI 2809 GACTC GAGTC(4/5)
957 GAGTC
SapI 1323 GAAGAGC GCTCTTC(1/4)
1452 GAAGAGC
SfiI 873 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2784 GGCCCCTAGGGCC
SmlI 331 CTTGAG C^T[CT][GA]AG
519 CTCGAG
XcmI 576 CCAGAAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1481 CCAACAAGGGCCTGG
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoII XmaCI XmaI