Restriction map for
pF1KSDA0155
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AviII BbeI
BbrPI BbvCI BcgI BfrI BglI
BglII BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspMI BssHII BstAPI BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FseI FspI HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NarI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 2951 GACGTC GACGT^C
AccI 348 GTATAC GT^[AC][GT]AC
AlwNI 865 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1678 ATTAAT AT^TAAT
AsnI 1678 ATTAAT AT^TAAT
Asp700I 1666 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 40 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3463 GGATCC G^GATCC
BclI 378 TGATCA T^GATCA
BsaAI 200 CACGTA [CT]AC^GT[GA]
BsaI 227 GAGACC GGTCTC(1/5)
BsmBI 2590 CGTCTC CGTCTC(1/5)
Bsp1407I 279 TGTACA T^GTACA
BspLU11I 2232 ACATGT A^CATGT
BsrBI 2512 GAGCGG CCGCTC(-3/-3)
BsrFI 3208 GCCGGC [GA]^CCGG[CT]
BsrGI 279 TGTACA T^GTACA
BssSI 1997 CTCGTG CACGAG(-5/-1)
Bst1107I 348 GTATAC GTA^TAC
BstZ17I 348 GTATAC GTA^TAC
Cfr10I 3208 GCCGGC [GA]^CCGG[CT]
DsaI 1616 CCATGG C^C[GA][CT]GG
EcoRI 1534 GAATTC G^AATTC
FbaI 378 TGATCA T^GATCA
HincII 1591 GTTGAC GT[CT]^[GA]AC
HindII 1591 GTTGAC GT[CT]^[GA]AC
NaeI 3208 GCCGGC GCC^GGC
NcoI 1616 CCATGG C^CATGG
NdeI 126 CATATG CA^TATG
NgoMIV 3208 GCCGGC G^CCGGC
NspI 2232 ACATGT [GA]CATG^[CT]
PflFI 40 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1743 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3233 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1678 ATTAAT AT^TAAT
SacI 542 GAGCTC GAGCT^C
SmlI 418 CTTGAG C^T[CT][GA]AG
SspBI 279 TGTACA T^GTACA
StuI 3476 AGGCCT AGG^CCT
Tth111I 40 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 720 TCTAGA T^CTAGA
XmnI 1666 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 5 CGCG CG^CG
1573 CGCG
AcyI 1425 GGCGTC G[GA]^CG[CT]C
2951 GACGTC
AflIII 1826 ACGTGT A^C[GA][CT]GT
2232 ACATGT
ApaI 2174 GGGCCC GGGCC^C
2633 GGGCCC
AvrII 1398 CCTAGG C^CTAGG
1608 CCTAGG
BalI 1898 TGGCCA TGG^CCA
3429 TGGCCA
BciVI 258 GGATAC GTATCC(6/5)
1221 GTATCC
BlnI 1398 CCTAGG C^CTAGG
1608 CCTAGG
BpmI 1349 CTCCAG CTGGAG(16/14)
2356 CTGGAG
BsaHI 1425 GGCGTC G[GA]^CG[CT]C
2951 GACGTC
BsaWI 72 ACCGGA [AT]^CCGG[AT]
3289 TCCGGT
BsrDI 566 CATTGC GCAATG(2/0)
616 CATTGC
BstUI 5 CGCG CG^CG
1573 CGCG
DraIII 1790 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2240 CACCCAGTG
EcoNI 414 CCTACTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1398 CCTAGGGGAGG
EcoT22I 881 ATGCAT ATGCA^T
1076 ATGCAT
HaeII 141 GGCGCT [GA]GCGC^[CT]
2095 AGCGCC
HgaI 1426 GCGTC GACGC(5/10)
1878 GCGTC
Hin1I 1425 GGCGTC G[GA]^CG[CT]C
2951 GACGTC
MluNI 1898 TGGCCA TGG^CCA
3429 TGGCCA
MscI 1898 TGGCCA TGG^CCA
3429 TGGCCA
MvnI 5 CGCG CG^CG
1573 CGCG
NheI 933 GCTAGC G^CTAGC
1812 GCTAGC
NsiI 881 ATGCAT ATGCA^T
1076 ATGCAT
SfcI 222 CTATAG C^T[GA][CT]AG
2432 CTACAG
SmaI 2171 CCCGGG CCC^GGG
2486 CCCGGG
XcmI 1380 CCATTTTAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3186 CCAGAACAAGTCTGG
XmaCI 2171 CCCGGG C^CCGGG
2486 CCCGGG
XmaI 2171 CCCGGG C^CCGGG
2486 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII