Restriction map for
pF1KSDA0161
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I FokI FseI
FspI HincII HindII HindIII HpaI
KasI Ksp632I KspI MaeI MamI
MfeI MflI MluI MroI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tth111I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 14 GGTACC G^GTACC
AccI 568 GTCTAC GT^[AC][GT]AC
AcsI 421 GAATTC [GA]^AATT[CT]
AcyI 336 GGCGTC G[GA]^CG[CT]C
AlwI 313 GATCC GGATC(4/5)
AlwNI 197 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 548 AGCGCT AGC^GCT
ApoI 421 GAATTC [GA]^AATT[CT]
Asp718I 14 GGTACC G^GTACC
AspHI 154 GAGCTC G[AT]GC[AT]^C
BanII 154 GAGCTC G[GA]GC[CT]^C
BclI 161 TGATCA T^GATCA
BsaHI 336 GGCGTC G[GA]^CG[CT]C
BsiHKAI 154 GAGCTC G[AT]GC[AT]^C
BsmBI 581 GAGACG CGTCTC(1/5)
BsrFI 17 ACCGGC [GA]^CCGG[CT]
BssHII 593 GCGCGC G^CGCGC
Cfr10I 17 ACCGGC [GA]^CCGG[CT]
Eco47III 548 AGCGCT AGC^GCT
EcoRI 421 GAATTC G^AATTC
EcoT22I 117 ATGCAT ATGCA^T
FauI 45 CCCGC CCCGC(4/6)
FbaI 161 TGATCA T^GATCA
HaeII 548 AGCGCT [GA]GCGC^[CT]
Hin1I 336 GGCGTC G[GA]^CG[CT]C
HinfI 646 GAGTC G^A[ACGT]TC
KpnI 14 GGTACC GGTAC^C
MaeII 371 ACGT A^CGT
NsiI 117 ATGCAT ATGCA^T
PflMI 366 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 646 GAGTC GAGTC(4/5)
PstI 379 CTGCAG CTGCA^G
SacI 154 GAGCTC GAGCT^C
Tsp45I 854 GTGAC ^GT[GC]AC
Van91I 366 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 23 CCACCTGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 537 CGCG CG^CG
594 CGCG
AciI 46 CCGC CCGC(-3/-1)
182 CCGC
AvaI 496 CCCGGG C^[CT]CG[GA]G
716 CCCGGG
AvaII 30 GGACC G^G[AT]CC
688 GGACC
BalI 456 TGGCCA TGG^CCA
809 TGGCCA
BmyI 154 GAGCTC G[AGT]GC[ACT]^C
330 GTGCCC
BpmI 364 CTCCAG CTGGAG(16/14)
643 CTGGAG
BsaI 472 GAGACC GGTCTC(1/5)
502 GAGACC
BsgI 397 GTGCAG GTGCAG(16/14)
843 GTGCAG
BsmFI 29 GGGAC GGGAC(10/14)
687 GGGAC
BsoBI 496 CCCGGG C^[CT]CG[GA]G
716 CCCGGG
Bsp1286I 154 GAGCTC G[AGT]GC[ACT]^C
330 GTGCCC
BspMI 343 ACCTGC ACCTGC(4/8)
433 ACCTGC
BstAPI 404 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
608 GCAAGAACTGC
BstUI 537 CGCG CG^CG
594 CGCG
DraII 29 GGGACCT [GA]G^G[ACGT]CC[CT]
687 GGGACCC
EaeI 456 TGGCCA [CT]^GGCC[GA]
809 TGGCCA
EcoO109I 29 GGGACCT [GA]G^G[ACGT]CC[CT]
687 GGGACCC
HgaI 535 GACGC GACGC(5/10)
337 GCGTC
MaeIII 854 GTGAC ^GT[ACGT]AC
867 GTTAC
MluNI 456 TGGCCA TGG^CCA
809 TGGCCA
MscI 456 TGGCCA TGG^CCA
809 TGGCCA
MvnI 537 CGCG CG^CG
594 CGCG
NlaIII 249 CATG CATG^
477 CATG
PpuMI 29 GGGACCT [GA]G^G[AT]CC[CT]
687 GGGACCC
PvuII 257 CAGCTG CAG^CTG
444 CAGCTG
SfcI 226 CTACAG C^T[GA][CT]AG
379 CTGCAG
SmaI 496 CCCGGG CCC^GGG
716 CCCGGG
TaqI 41 TCGA T^CGA
575 TCGA
TthHB8I 41 TCGA T^CGA
575 TCGA
XmaCI 496 CCCGGG C^CCGGG
716 CCCGGG
XmaI 496 CCCGGG C^CCGGG
716 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI BanI BbvI BcnI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DpnII EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp509I TspRI