Restriction map for
pF1KSDA0163
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BanII BbrPI BbvCI BciVI
BclI BfrI BglII BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MamI MfeI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 391 GTCTAC GT^[AC][GT]AC
AocI 859 CCTAAGG CC^T[ACGT]AGG
AvaI 514 CCCGAG C^[CT]CG[GA]G
AvrII 333 CCTAGG C^CTAGG
BalI 1031 TGGCCA TGG^CCA
BcgI 309 GCAGCCCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 334 CTAG C^TAG
BlnI 333 CCTAGG C^CTAGG
BpmI 1477 CTGGAG CTGGAG(16/14)
BsgI 885 CTGCAC GTGCAG(16/14)
BsiEI 1176 CGGCCG CG[GA][CT]^CG
BsmI 616 GCATTC GAATGC(1/-1)
BsoBI 514 CCCGAG C^[CT]CG[GA]G
BspLU11I 808 ACATGT A^CATGT
BsrFI 1106 ACCGGC [GA]^CCGG[CT]
BstAPI 1482 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 859 CCTAAGG CC^T[ACGT]AGG
Cfr10I 1106 ACCGGC [GA]^CCGG[CT]
DraIII 292 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1176 CGGCCG C^GGCCG
EclXI 1176 CGGCCG C^GGCCG
Eco52I 1176 CGGCCG C^GGCCG
Eco81I 859 CCTAAGG CC^T[ACGT]AGG
EcoNI 492 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 219 GCGTC GACGC(5/10)
HincII 814 GTTGAC GT[CT]^[GA]AC
HindII 814 GTTGAC GT[CT]^[GA]AC
KspI 250 CCGCGG CCGC^GG
MaeI 334 CTAG C^TAG
MluNI 1031 TGGCCA TGG^CCA
MscI 1031 TGGCCA TGG^CCA
MslI 785 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 808 ACATGT [GA]CATG^[CT]
PpuMI 965 AGGACCC [GA]G^G[AT]CC[CT]
SacII 250 CCGCGG CCGC^GG
StuI 1355 AGGCCT AGG^CCT
TaqI 1136 TCGA T^CGA
TthHB8I 1136 TCGA T^CGA
XcmI 435 CCAGCGCCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 957 GTAC GT^AC
1311 GTAC
AflIII 689 ACGTGT A^C[GA][CT]GT
808 ACATGT
AspHI 49 GTGCTC G[AT]GC[AT]^C
1581 GAGCAC
BamHI 246 GGATCC G^GATCC
1184 GGATCC
BbsI 1522 GAAGAC GAAGAC(2/6)
780 GTCTTC
BpuAI 1522 GAAGAC GAAGAC(2/6)
780 GTCTTC
BseRI 1065 CTCCTC GAGGAG(10/8)
269 GAGGAG
BsiHKAI 49 GTGCTC G[AT]GC[AT]^C
1581 GAGCAC
BspMI 430 ACCTGC ACCTGC(4/8)
889 ACCTGC
BssSI 46 CTCGTG CACGAG(-5/-1)
1552 CTCGTG
BstYI 246 GGATCC [GA]^GATC[CT]
1184 GGATCC
DpnII 247 GATC ^GATC
1185 GATC
MboI 247 GATC ^GATC
1185 GATC
MflI 246 GGATCC [GA]^GATC[CT]
1184 GGATCC
NcoI 894 CCATGG C^CATGG
1028 CCATGG
NdeII 247 GATC ^GATC
1185 GATC
PflMI 1432 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1606 CCAGGGGGTGG
PstI 1391 CTGCAG CTGCA^G
1592 CTGCAG
RsaI 957 GTAC GT^AC
1311 GTAC
Sau3AI 247 GATC ^GATC
1185 GATC
SfcI 1391 CTGCAG C^T[GA][CT]AG
1592 CTGCAG
Van91I 1432 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1606 CCAGGGGGTGG
XhoII 246 GGATCC [GA]^GATC[CT]
1184 GGATCC
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AvaII BanI BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NlaIII NlaIV PleI
PvuII SapI Sau96I ScrFI SfaNI
SfoI StyI TseI Tsp45I TspRI