Restriction map for
pF1KSDA0167
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BciVI
BfrI BglII BlnI BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstBI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoRV FseI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MslI MunI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 1742 TCCGGA T^CCGGA
AocI 84 CCTCAGG CC^T[ACGT]AGG
ApaI 1781 GGGCCC GGGCC^C
Asp700I 444 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1794 CACGTG CAC^GTG
BcgI 867 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 167 TGATCA T^GATCA
BlpI 2070 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2070 GCTGAGC GC^T[ACGT]AGC
BsaAI 1794 CACGTG [CT]AC^GT[GA]
BsaI 1436 GAGACC GGTCTC(1/5)
BseAI 1742 TCCGGA T^CCGGA
BsiEI 2225 CGGCCG CG[GA][CT]^CG
BsmBI 466 CGTCTC CGTCTC(1/5)
BsmI 152 GCATTC GAATGC(1/-1)
BspEI 1742 TCCGGA T^CCGGA
BspMI 1376 ACCTGC ACCTGC(4/8)
BsrDI 1671 GCAATG GCAATG(2/0)
BssSI 581 CTCGTG CACGAG(-5/-1)
BstYI 1655 AGATCC [GA]^GATC[CT]
Bsu36I 84 CCTCAGG CC^T[ACGT]AGG
CelII 2070 GCTGAGC GC^T[ACGT]AGC
EagI 2225 CGGCCG C^GGCCG
EclXI 2225 CGGCCG C^GGCCG
Eco52I 2225 CGGCCG C^GGCCG
Eco81I 84 CCTCAGG CC^T[ACGT]AGG
EcoRI 1758 GAATTC G^AATTC
EcoT22I 1 ATGCAT ATGCA^T
FbaI 167 TGATCA T^GATCA
HindIII 975 AAGCTT A^AGCTT
MflI 1655 AGATCC [GA]^GATC[CT]
MroI 1742 TCCGGA T^CCGGA
NheI 2470 GCTAGC G^CTAGC
NsiI 1 ATGCAT ATGCA^T
NspI 3 GCATGC [GA]CATG^[CT]
PmaCI 1794 CACGTG CAC^GTG
PmlI 1794 CACGTG CAC^GTG
SexAI 284 ACCAGGT A^CC[AT]GGT
SphI 3 GCATGC GCATG^C
XcmI 1802 CCAGCTTGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1655 AGATCC [GA]^GATC[CT]
XmnI 444 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 1395 GAATTT [GA]^AATT[CT]
1758 GAATTC
ApoI 1395 GAATTT [GA]^AATT[CT]
1758 GAATTC
AviII 698 TGCGCA TGC^GCA
1700 TGCGCA
BbeI 1816 GGCGCC GGCGC^C
2064 GGCGCC
BbvCI 1238 CCTCAGC CCTCAGC(-5/-2)
1410 CCTCAGC
BsgI 907 CTGCAC GTGCAG(16/14)
2116 GTGCAG
BstAPI 379 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2253 GCAACTGCTGC
BstEII 690 GGTGACC G^GT[ACGT]ACC
847 GGTCACC
BstPI 690 GGTGACC G^GT[ACGT]ACC
847 GGTCACC
EcoO65I 690 GGTGACC G^GT[ACGT]ACC
847 GGTCACC
FspI 698 TGCGCA TGC^GCA
1700 TGCGCA
HgaI 2293 GACGC GACGC(5/10)
2488 GACGC
KasI 1816 GGCGCC G^GCGCC
2064 GGCGCC
MseI 1133 TTAA T^TAA
1250 TTAA
NarI 1816 GGCGCC GG^CGCC
2064 GGCGCC
NcoI 471 CCATGG C^CATGG
1475 CCATGG
PpuMI 901 GGGTCCC [GA]G^G[AT]CC[CT]
2192 AGGACCC
SfoI 1816 GGCGCC GGC^GCC
2064 GGCGCC
SmaI 1197 CCCGGG CCC^GGG
2376 CCCGGG
Tru9I 1133 TTAA T^TAA
1250 TTAA
XmaCI 1197 CCCGGG C^CCGGG
2376 CCCGGG
XmaI 1197 CCCGGG C^CCGGG
2376 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I