Restriction map for
pF1KSDA0170
##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI AspEI AspI AviII
BalI BbeI BbrPI BciVI BsaAI
BsaHI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssHII BssSI
BstAPI BstBI BstUI ClaI CpoI
DraI EaeI EagI Eam1105I EclXI
Eco52I EcoRV FseI FspI Hin1I
HpaI KasI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 2221 GGTACC G^GTACC
AccI 2444 GTATAC GT^[AC][GT]AC
AcsI 5095 GAATTC [GA]^AATT[CT]
AflII 1843 CTTAAG C^TTAAG
Aor51HI 1236 AGCGCT AGC^GCT
ApoI 5095 GAATTC [GA]^AATT[CT]
Asp718I 2221 GGTACC G^GTACC
AvrII 2344 CCTAGG C^CTAGG
BcgI 987 CGACACTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 6116 TGATCA T^GATCA
BfrI 1843 CTTAAG C^TTAAG
BlnI 2344 CCTAGG C^CTAGG
BlpI 961 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 961 GCTCAGC GC^T[ACGT]AGC
BsaBI 231 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3645 CGACCG CG[GA][CT]^CG
BsmBI 4409 CGTCTC CGTCTC(1/5)
BspHI 1860 TCATGA T^CATGA
BspLU11I 2028 ACATGT A^CATGT
BsrBI 5716 GAGCGG CCGCTC(-3/-3)
BsrFI 5076 GCCGGT [GA]^CCGG[CT]
Bst1107I 2444 GTATAC GTA^TAC
BstEII 5907 GGTGACC G^GT[ACGT]ACC
BstPI 5907 GGTGACC G^GT[ACGT]ACC
BstZ17I 2444 GTATAC GTA^TAC
CelII 961 GCTCAGC GC^T[ACGT]AGC
CfoI 1237 GCGC GCG^C
Cfr10I 5076 GCCGGT [GA]^CCGG[CT]
DraIII 4788 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1236 AGCGCT AGC^GCT
EcoNI 2476 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5907 GGTGACC G^GT[ACGT]ACC
EcoRI 5095 GAATTC G^AATTC
EcoT22I 1606 ATGCAT ATGCA^T
FbaI 6116 TGATCA T^GATCA
HaeII 1236 AGCGCT [GA]GCGC^[CT]
HgaI 5953 GACGC GACGC(5/10)
HhaI 1237 GCGC GCG^C
HinP1I 1237 GCGC G^CGC
HincII 2169 GTTGAC GT[CT]^[GA]AC
HindII 2169 GTTGAC GT[CT]^[GA]AC
HindIII 1184 AAGCTT A^AGCTT
KpnI 2221 GGTACC GGTAC^C
MamI 231 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4709 CAATTG C^AATTG
MunI 4709 CAATTG C^AATTG
NsiI 1606 ATGCAT ATGCA^T
PaeR7I 4240 CTCGAG C^TCGAG
RcaI 1860 TCATGA T^CATGA
SexAI 5774 ACCTGGT A^CC[AT]GGT
SmaI 422 CCCGGG CCC^GGG
SphI 6083 GCATGC GCATG^C
XbaI 1698 TCTAGA T^CTAGA
XhoI 4240 CTCGAG C^TCGAG
XmaCI 422 CCCGGG C^CCGGG
XmaI 422 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AflIII 1311 ACGTGT A^C[GA][CT]GT
2028 ACATGT
Asp700I 355 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1050 GAAGATCTTC
BglI 87 GCCAGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1153 GCCCCACAGGC
BsmI 173 GAATGC GAATGC(1/-1)
600 GCATTC
BspMI 3036 ACCTGC ACCTGC(4/8)
5380 GCAGGT
BsrDI 2200 CATTGC GCAATG(2/0)
6142 CATTGC
SapI 1378 GAAGAGC GCTCTTC(1/4)
5685 GCTCTTC
TaqI 980 TCGA T^CGA
4241 TCGA
TthHB8I 980 TCGA T^CGA
4241 TCGA
XmnI 355 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1050 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DrdI DsaI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII