Restriction map for
pF1KSDA0180
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaI
BseAI BsgI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
DsaI Eam1105I Eco47III EcoNI EcoRI
EcoRV EcoT22I FbaI FseI HgaI
HindIII HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SbfI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tsp45I Tsp509I
Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 12 CGCG CG^CG
AcyI 4 GGCGCC G[GA]^CG[CT]C
AocI 1000 CCTGAGG CC^T[ACGT]AGG
Asp700I 264 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 952 GAGCTC G[AT]GC[AT]^C
AvaI 63 CCCGGG C^[CT]CG[GA]G
AviII 716 TGCGCA TGC^GCA
BbeI 4 GGCGCC GGCGC^C
BfaI 604 CTAG C^TAG
BglI 10 GCCGCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 27 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 27 GCTGAGC GC^T[ACGT]AGC
BsaHI 4 GGCGCC G[GA]^CG[CT]C
BsaWI 561 TCCGGT [AT]^CCGG[AT]
BseRI 1065 CTCCTC GAGGAG(10/8)
BsiHKAI 952 GAGCTC G[AT]GC[AT]^C
BsmAI 1063 GTCTC GTCTC(1/5)
BsmI 707 GCATTC GAATGC(1/-1)
BsoBI 63 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1022 TGTACA T^GTACA
BspMI 101 ACCTGC ACCTGC(4/8)
BsrFI 94 GCCGGT [GA]^CCGG[CT]
BsrGI 1022 TGTACA T^GTACA
BstEII 97 GGTTACC G^GT[ACGT]ACC
BstPI 97 GGTTACC G^GT[ACGT]ACC
BstUI 12 CGCG CG^CG
BstXI 1044 CCACTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 866 AGATCC [GA]^GATC[CT]
Bsu36I 1000 CCTGAGG CC^T[ACGT]AGG
CelII 27 GCTGAGC GC^T[ACGT]AGC
Cfr10I 94 GCCGGT [GA]^CCGG[CT]
EarI 964 CTCTTC CTCTTC(1/4)
Eco81I 1000 CCTGAGG CC^T[ACGT]AGG
EcoO65I 97 GGTTACC G^GT[ACGT]ACC
FspI 716 TGCGCA TGC^GCA
Hin1I 4 GGCGCC G[GA]^CG[CT]C
HincII 1015 GTCGAC GT[CT]^[GA]AC
HindII 1015 GTCGAC GT[CT]^[GA]AC
KasI 4 GGCGCC G^GCGCC
Ksp632I 964 CTCTTC CTCTTC(1/4)
MaeI 604 CTAG C^TAG
MaeIII 98 GTTAC ^GT[ACGT]AC
MflI 866 AGATCC [GA]^GATC[CT]
MvnI 12 CGCG CG^CG
NarI 4 GGCGCC GG^CGCC
PflMI 341 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 767 CTGCAG CTGCA^G
SacI 952 GAGCTC GAGCT^C
SalI 1015 GTCGAC G^TCGAC
SapI 963 GCTCTTC GCTCTTC(1/4)
ScaI 266 AGTACT AGT^ACT
SfoI 4 GGCGCC GGC^GCC
SmaI 63 CCCGGG CCC^GGG
SspBI 1022 TGTACA T^GTACA
TaqI 1016 TCGA T^CGA
TthHB8I 1016 TCGA T^CGA
Van91I 341 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 866 AGATCC [GA]^GATC[CT]
XmaCI 63 CCCGGG C^CCGGG
XmaI 63 CCCGGG C^CCGGG
XmnI 264 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 916 GTCTAC GT^[AC][GT]AC
1015 GTCGAC
AlwI 867 GATCC GGATC(4/5)
456 GGATC
AvaII 87 GGACC G^G[AT]CC
1098 GGACC
BanI 4 GGCGCC G^G[CT][GA]CC
336 GGCACC
BsiEI 22 CGGCCG CG[GA][CT]^CG
92 CGGCCG
BsrBI 58 CCGCTC CCGCTC(-3/-3)
1093 GAGCGG
EagI 22 CGGCCG C^GGCCG
92 CGGCCG
EclXI 22 CGGCCG C^GGCCG
92 CGGCCG
Eco52I 22 CGGCCG C^GGCCG
92 CGGCCG
EcoT14I 191 CCTTGG C^C[AT][AT]GG
205 CCTTGG
HaeII 4 GGCGCC [GA]GCGC^[CT]
125 GGCGCT
HinfI 675 GATTC G^A[ACGT]TC
931 GATTC
MaeII 415 ACGT A^CGT
920 ACGT
MspA1I 25 CCGCTG C[AC]G^C[GT]G
821 CAGCGG
PpuMI 86 GGGACCC [GA]G^G[AT]CC[CT]
1097 GGGACCT
StyI 191 CCTTGG C^C[AT][AT]GG
205 CCTTGG
TfiI 675 GATTC G^A[AT]TC
931 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI