Restriction map for
pF1KSDA0181
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII AccII AccIII AflII AgeI
Alw44I Aor51HI ApaLI AscI AspI
AviII BbrPI BciVI BfrI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FseI FspI HindIII
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PvuI RsrII SacII SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Acc65I 5763 GGTACC G^GTACC
AccI 5186 GTCGAC GT^[AC][GT]AC
AclI 4708 AACGTT AA^CGTT
AflIII 3456 ACATGT A^C[GA][CT]GT
AseI 3766 ATTAAT AT^TAAT
AsnI 3766 ATTAAT AT^TAAT
Asp700I 3317 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5763 GGTACC G^GTACC
BbeI 2076 GGCGCC GGCGC^C
BspHI 3110 TCATGA T^CATGA
BspLU11I 3456 ACATGT A^CATGT
BsrBI 316 GAGCGG CCGCTC(-3/-3)
BsrDI 646 GCAATG GCAATG(2/0)
BssSI 5207 CACGAG CACGAG(-5/-1)
DraI 3795 TTTAAA TTT^AAA
EcoNI 5844 CCTAGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 2076 GGCGCC [GA]GCGC^[CT]
HgaI 1101 GACGC GACGC(5/10)
HpaI 1336 GTTAAC GTT^AAC
KasI 2076 GGCGCC G^GCGCC
KpnI 5763 GGTACC GGTAC^C
NarI 2076 GGCGCC GG^CGCC
NheI 5311 GCTAGC G^CTAGC
PshAI 2279 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3766 ATTAAT AT^TAAT
Psp1406I 4708 AACGTT AA^CGTT
RcaI 3110 TCATGA T^CATGA
SalI 5186 GTCGAC G^TCGAC
ScaI 3462 AGTACT AGT^ACT
SexAI 2910 ACCAGGT A^CC[AT]GGT
SfiI 2366 GGCCACCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2076 GGCGCC GGC^GCC
XmnI 3317 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 1101 GACGCC G[GA]^CG[CT]C
2076 GGCGCC
AhdI 5189 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5391 GACCAATAGTC
AspEI 5189 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5391 GACCAATAGTC
AvrII 1521 CCTAGG C^CTAGG
3784 CCTAGG
BalI 4142 TGGCCA TGG^CCA
5469 TGGCCA
BcgI 1577 CGAACTTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4459 GCACCAACATCG
BclI 2829 TGATCA T^GATCA
3810 TGATCA
BglII 2784 AGATCT A^GATCT
4533 AGATCT
BlnI 1521 CCTAGG C^CTAGG
3784 CCTAGG
BsaHI 1101 GACGCC G[GA]^CG[CT]C
2076 GGCGCC
BsrFI 2903 ACCGGC [GA]^CCGG[CT]
5417 ACCGGC
CfoI 2077 GCGC GCG^C
3604 GCGC
Cfr10I 2903 ACCGGC [GA]^CCGG[CT]
5417 ACCGGC
Eam1105I 5189 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5391 GACCAATAGTC
FbaI 2829 TGATCA T^GATCA
3810 TGATCA
HhaI 2077 GCGC GCG^C
3604 GCGC
Hin1I 1101 GACGCC G[GA]^CG[CT]C
2076 GGCGCC
HinP1I 2077 GCGC G^CGC
3604 GCGC
MluNI 4142 TGGCCA TGG^CCA
5469 TGGCCA
MscI 4142 TGGCCA TGG^CCA
5469 TGGCCA
NcoI 455 CCATGG C^CATGG
2544 CCATGG
SapI 6061 GAAGAGC GCTCTTC(1/4)
4751 GCTCTTC
SphI 1668 GCATGC GCATG^C
1868 GCATGC
SspI 201 AATATT AAT^ATT
3769 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI