Restriction map for
pF1KSDA0195
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BfrI BlnI
BsaAI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI Eco47III
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tsp509I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 2554 GTCTAC GT^[AC][GT]AC
AccII 56 CGCG CG^CG
AhdI 515 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 515 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 2291 GCAAGTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2054 TGATCA T^GATCA
BfaI 2911 CTAG C^TAG
BglII 2462 AGATCT A^GATCT
BsaBI 2958 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3707 CGGCCG CG[GA][CT]^CG
BsmI 291 GAATGC GAATGC(1/-1)
BstUI 56 CGCG CG^CG
DrdI 3883 GACAGCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3707 CGGCCG C^GGCCG
Eam1105I 515 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3707 CGGCCG C^GGCCG
Eco52I 3707 CGGCCG C^GGCCG
FbaI 2054 TGATCA T^GATCA
MaeI 2911 CTAG C^TAG
MamI 2958 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2069 TTAA T^TAA
MvnI 56 CGCG CG^CG
NaeI 868 GCCGGC GCC^GGC
NcoI 2106 CCATGG C^CATGG
NgoMIV 868 GCCGGC G^CCGGC
PflMI 3794 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1340 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 3683 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1582 GAAGAGC GCTCTTC(1/4)
SexAI 518 ACCTGGT A^CC[AT]GGT
Tru9I 2069 TTAA T^TAA
Van91I 3794 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 961 GGCGTC G[GA]^CG[CT]C
1399 GACGCC
AocI 414 CCTCAGG CC^T[ACGT]AGG
1158 CCTGAGG
BalI 576 TGGCCA TGG^CCA
1270 TGGCCA
BamHI 1259 GGATCC G^GATCC
3063 GGATCC
BsaHI 961 GGCGTC G[GA]^CG[CT]C
1399 GACGCC
BsmBI 509 GAGACG CGTCTC(1/5)
1397 GAGACG
BsrDI 1833 CATTGC GCAATG(2/0)
3054 CATTGC
BstEII 2985 GGTGACC G^GT[ACGT]ACC
3963 GGTGACC
BstPI 2985 GGTGACC G^GT[ACGT]ACC
3963 GGTGACC
Bsu36I 414 CCTCAGG CC^T[ACGT]AGG
1158 CCTGAGG
DraIII 2337 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
2866 CACCAAGTG
Eco81I 414 CCTCAGG CC^T[ACGT]AGG
1158 CCTGAGG
EcoO65I 2985 GGTGACC G^GT[ACGT]ACC
3963 GGTGACC
EcoT14I 1928 CCAAGG C^C[AT][AT]GG
2106 CCATGG
Hin1I 961 GGCGTC G[GA]^CG[CT]C
1399 GACGCC
HincII 523 GTCAAC GT[CT]^[GA]AC
2533 GTCAAC
HindII 523 GTCAAC GT[CT]^[GA]AC
2533 GTCAAC
MluNI 576 TGGCCA TGG^CCA
1270 TGGCCA
MscI 576 TGGCCA TGG^CCA
1270 TGGCCA
SacI 2397 GAGCTC GAGCT^C
2575 GAGCTC
SmlI 736 CTTGAG C^T[CT][GA]AG
3520 CTCAAG
SphI 2441 GCATGC GCATG^C
3249 GCATGC
StyI 1928 CCAAGG C^C[AT][AT]GG
2106 CCATGG
TaqI 2300 TCGA T^CGA
3236 TCGA
TthHB8I 2300 TCGA T^CGA
3236 TCGA
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI TfiI TseI Tsp45I TspRI
XcmI XhoII XmaCI XmaI