Restriction map for
pF1KSDA0198
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BpmI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MroI MseI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 704 CGCG CG^CG
AcsI 128 AAATTT [GA]^AATT[CT]
AcyI 428 GGCGCC G[GA]^CG[CT]C
ApoI 128 AAATTT [GA]^AATT[CT]
BbeI 428 GGCGCC GGCGC^C
BbsI 1432 GTCTTC GAAGAC(2/6)
BpuAI 1432 GTCTTC GAAGAC(2/6)
BsaHI 428 GGCGCC G[GA]^CG[CT]C
BsaI 195 GAGACC GGTCTC(1/5)
BseRI 52 GAGGAG GAGGAG(10/8)
BsiEI 588 CGACCG CG[GA][CT]^CG
BsmI 1480 GCATTC GAATGC(1/-1)
BspLU11I 764 ACATGT A^CATGT
BspMI 1271 ACCTGC ACCTGC(4/8)
BstEII 460 GGTGACC G^GT[ACGT]ACC
BstPI 460 GGTGACC G^GT[ACGT]ACC
BstUI 704 CGCG CG^CG
BstXI 675 CCAGCGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1033 GGATCT [GA]^GATC[CT]
DraIII 625 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1206 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 460 GGTGACC G^GT[ACGT]ACC
EcoT22I 442 ATGCAT ATGCA^T
HaeII 428 GGCGCC [GA]GCGC^[CT]
Hin1I 428 GGCGCC G[GA]^CG[CT]C
KasI 428 GGCGCC G^GCGCC
MflI 1033 GGATCT [GA]^GATC[CT]
MluI 703 ACGCGT A^CGCGT
MvnI 704 CGCG CG^CG
NaeI 1149 GCCGGC GCC^GGC
NarI 428 GGCGCC GG^CGCC
NgoMIV 1149 GCCGGC G^CCGGC
NsiI 442 ATGCAT ATGCA^T
PleI 105 GAGTC GAGTC(4/5)
ScaI 665 AGTACT AGT^ACT
SfaNI 40 GATGC GCATC(5/9)
SfoI 428 GGCGCC GGC^GCC
SpeI 72 ACTAGT A^CTAGT
SphI 444 GCATGC GCATG^C
XhoII 1033 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 703 ACGCGT A^C[GA][CT]GT
764 ACATGT
Alw44I 634 GTGCAC G^TGCAC
943 GTGCAC
AlwI 361 GATCC GGATC(4/5)
1033 GGATC
ApaLI 634 GTGCAC G^TGCAC
943 GTGCAC
AvaI 86 CTCGGG C^[CT]CG[GA]G
839 CTCGGG
BalI 266 TGGCCA TGG^CCA
437 TGGCCA
BcnI 93 CCGGG CC^[GC]GG
540 CCGGG
BsgI 217 CTGCAC GTGCAG(16/14)
1414 CTGCAC
BsmAI 195 GAGAC GTCTC(1/5)
1110 GTCTC
BsoBI 86 CTCGGG C^[CT]CG[GA]G
839 CTCGGG
CfoI 429 GCGC GCG^C
1223 GCGC
HhaI 429 GCGC GCG^C
1223 GCGC
HinP1I 429 GCGC G^CGC
1223 GCGC
MaeII 845 ACGT A^CGT
1438 ACGT
MluNI 266 TGGCCA TGG^CCA
437 TGGCCA
MscI 266 TGGCCA TGG^CCA
437 TGGCCA
NciI 93 CCGGG CC^[GC]GG
540 CCGGG
NcoI 881 CCATGG C^CATGG
959 CCATGG
PflMI 1245 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1381 CCAGGAGCTGG
SapI 717 GAAGAGC GCTCTTC(1/4)
808 GAAGAGC
SmlI 739 CTCAAG C^T[CT][GA]AG
1370 CTCAAG
Tsp45I 461 GTGAC ^GT[GC]AC
578 GTGAC
Van91I 1245 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1381 CCAGGAGCTGG
XcmI 1064 CCAAAGTGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1298 CCACAGGAGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BanII BbvCI BbvI
BfaI BlpI BmyI Bpu1102I BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CelII Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI StuI StyI TfiI TseI
Tsp509I TspRI