Restriction map for
pF1KSDA0200
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AlwI AscI Asp700I
Asp718I AspI AviII BamHI BbrPI
BcgI BciVI BfrI BglII BsaAI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I ClaI DraI DraIII DrdI
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI MamI MfeI MflI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PshAI
Psp1406I PvuI SalI SapI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AgeI 2469 ACCGGT A^CCGGT
AhdI 838 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2720 GTGCAC G^TGCAC
AocI 2137 CCTCAGG CC^T[ACGT]AGG
Aor51HI 182 AGCGCT AGC^GCT
ApaLI 2720 GTGCAC G^TGCAC
AseI 886 ATTAAT AT^TAAT
AsnI 886 ATTAAT AT^TAAT
AspEI 838 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1020 CCTAGG C^CTAGG
BbvCI 2770 CCTCAGC CCTCAGC(-5/-2)
BclI 680 TGATCA T^GATCA
BlnI 1020 CCTAGG C^CTAGG
BlpI 2681 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2681 GCTCAGC GC^T[ACGT]AGC
BsaI 3041 GGTCTC GGTCTC(1/5)
BsaWI 2469 ACCGGT [AT]^CCGG[AT]
BsmBI 2376 CGTCTC CGTCTC(1/5)
BsmI 2528 GAATGC GAATGC(1/-1)
BssHII 207 GCGCGC G^CGCGC
BssSI 1654 CACGAG CACGAG(-5/-1)
BstXI 1044 CCACACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2137 CCTCAGG CC^T[ACGT]AGG
CelII 2681 GCTCAGC GC^T[ACGT]AGC
CpoI 2890 CGGACCG CG^G[AT]CCG
EagI 300 CGGCCG C^GGCCG
Eam1105I 838 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 450 CTCTTC CTCTTC(1/4)
EclXI 300 CGGCCG C^GGCCG
Eco47III 182 AGCGCT AGC^GCT
Eco52I 300 CGGCCG C^GGCCG
Eco81I 2137 CCTCAGG CC^T[ACGT]AGG
FbaI 680 TGATCA T^GATCA
Ksp632I 450 CTCTTC CTCTTC(1/4)
PaeR7I 2126 CTCGAG C^TCGAG
PinAI 2469 ACCGGT A^CCGGT
PshBI 886 ATTAAT AT^TAAT
RsrII 2890 CGGACCG CG^G[AT]CCG
SgrAI 70 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 2126 CTCGAG C^T[CT][GA]AG
SspI 889 AATATT AAT^ATT
StuI 2876 AGGCCT AGG^CCT
XhoI 2126 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 901 GAATTC [GA]^AATT[CT]
2505 GAATTC
AfaI 1004 GTAC GT^AC
2016 GTAC
ApoI 901 GAATTC [GA]^AATT[CT]
2505 GAATTC
BbeI 92 GGCGCC GGCGC^C
249 GGCGCC
BsiEI 300 CGGCCG CG[GA][CT]^CG
2850 CGGTCG
BsmAI 2377 GTCTC GTCTC(1/5)
3042 GTCTC
BspHI 321 TCATGA T^CATGA
1394 TCATGA
BstAPI 1869 GCATCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2775 GCAGGGCTTGC
EcoRI 901 GAATTC G^AATTC
2505 GAATTC
HphI 1473 TCACC GGTGA(8/7)
2485 TCACC
KasI 92 GGCGCC G^GCGCC
249 GGCGCC
KspI 40 CCGCGG CCGC^GG
2894 CCGCGG
MaeII 2357 ACGT A^CGT
2567 ACGT
MslI 316 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
589 CACTTGAATG
NarI 92 GGCGCC GG^CGCC
249 GGCGCC
RcaI 321 TCATGA T^CATGA
1394 TCATGA
RsaI 1004 GTAC GT^AC
2016 GTAC
SacI 757 GAGCTC GAGCT^C
2679 GAGCTC
SacII 40 CCGCGG CCGC^GG
2894 CCGCGG
SfoI 92 GGCGCC GGC^GCC
249 GGCGCC
XcmI 11 CCACCTGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2657 CCAACAGGCCCATGG
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI MaeI MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmaCI XmaI