Restriction map for
pF1KSDA0214
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# NO CUT ENZYMES ( 95 enzymes) #
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Acc65I AccII AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbrPI BciVI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspEI BspHI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstYI BstZ17I Bsu36I CelII DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FseI FspI
HindIII HpaI KpnI KspI MamI
MflI MluI MroI MvnI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SacII SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1290 GTCGAC GT^[AC][GT]AC
AflII 85 CTTAAG C^TTAAG
ApaI 1671 GGGCCC GGGCC^C
BanI 1316 GGCGCC G^G[CT][GA]CC
BbeI 1316 GGCGCC GGCGC^C
BbvCI 64 GCTGAGG CCTCAGC(-5/-2)
BcgI 23 GCAACTCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 335 TGATCA T^GATCA
BfaI 532 CTAG C^TAG
BfrI 85 CTTAAG C^TTAAG
BsaAI 177 CACGTA [CT]AC^GT[GA]
BsmBI 739 CGTCTC CGTCTC(1/5)
BspDI 878 ATCGAT AT^CGAT
BsrBI 1186 GAGCGG CCGCTC(-3/-3)
BstAPI 1470 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 878 ATCGAT AT^CGAT
CpoI 1085 CGGTCCG CG^G[AT]CCG
DraIII 724 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1540 GACATGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 402 GCGGG CCCGC(4/6)
FbaI 335 TGATCA T^GATCA
KasI 1316 GGCGCC G^GCGCC
MaeI 532 CTAG C^TAG
MfeI 390 CAATTG C^AATTG
MunI 390 CAATTG C^AATTG
NaeI 523 GCCGGC GCC^GGC
NarI 1316 GGCGCC GG^CGCC
NgoMIV 523 GCCGGC G^CCGGC
RsrII 1085 CGGTCCG CG^G[AT]CCG
SalI 1290 GTCGAC G^TCGAC
SexAI 1712 ACCAGGT A^CC[AT]GGT
SfoI 1316 GGCGCC GGC^GCC
SmaI 2117 CCCGGG CCC^GGG
StuI 428 AGGCCT AGG^CCT
TfiI 939 GATTC G^A[AT]TC
XmaCI 2117 CCCGGG C^CCGGG
XmaI 2117 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 229 GACGTC GACGT^C
2110 GACGTC
AcsI 1017 GAATTT [GA]^AATT[CT]
1204 AAATTT
AlwI 881 GATCC GGATC(4/5)
876 GGATC
ApoI 1017 GAATTT [GA]^AATT[CT]
1204 AAATTT
AspI 1418 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1711 GACCAGGTC
BsgI 1135 CTGCAC GTGCAG(16/14)
1396 CTGCAC
BsmAI 54 GAGAC GTCTC(1/5)
740 GTCTC
BspLU11I 1430 ACATGT A^CATGT
2237 ACATGT
BstEII 238 GGTTACC G^GT[ACGT]ACC
1809 GGTTACC
BstPI 238 GGTTACC G^GT[ACGT]ACC
1809 GGTTACC
BstXI 617 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
845 CCAGCTTCCTGG
EcoO65I 238 GGTTACC G^GT[ACGT]ACC
1809 GGTTACC
EcoT22I 74 ATGCAT ATGCA^T
782 ATGCAT
HincII 1290 GTCGAC GT[CT]^[GA]AC
2107 GTTGAC
HindII 1290 GTCGAC GT[CT]^[GA]AC
2107 GTTGAC
MseI 86 TTAA T^TAA
1256 TTAA
NcoI 1805 CCATGG C^CATGG
1844 CCATGG
NsiI 74 ATGCAT ATGCA^T
782 ATGCAT
PflFI 1418 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1711 GACCAGGTC
PflMI 344 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1989 CCAGTTTGTGG
PpuMI 1837 AGGACCT [GA]G^G[AT]CC[CT]
2080 GGGACCT
SacI 1225 GAGCTC GAGCT^C
2230 GAGCTC
Tru9I 86 TTAA T^TAA
1256 TTAA
Tth111I 1418 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1711 GACCAGGTC
Van91I 344 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1989 CCAGTTTGTGG
XcmI 845 CCAGCTTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1835 CCAGGACCTCCATGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwNI Asp700I AspHI AvaI AvaII
BalI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI