Restriction map for
pF1KSDA0218
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AccIII AclI
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BbeI BbrPI BcgI
BciVI BclI BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BspEI BspMI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MluI MluNI MroI MscI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
AflIII 2018 ACATGT A^C[GA][CT]GT
Alw44I 1803 GTGCAC G^TGCAC
AlwNI 725 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1803 GTGCAC G^TGCAC
AspI 1096 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 340 GGATCC G^GATCC
BanI 1751 GGCACC G^G[CT][GA]CC
BbsI 330 GTCTTC GAAGAC(2/6)
BbvCI 469 GCTGAGG CCTCAGC(-5/-2)
BpuAI 330 GTCTTC GAAGAC(2/6)
BsaBI 1903 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaI 536 GAGACC GGTCTC(1/5)
Bsp1407I 1121 TGTACA T^GTACA
BspDI 591 ATCGAT AT^CGAT
BspHI 1922 TCATGA T^CATGA
BspLU11I 2018 ACATGT A^CATGT
BsrBI 10 GAGCGG CCGCTC(-3/-3)
BsrDI 1963 CATTGC GCAATG(2/0)
BsrGI 1121 TGTACA T^GTACA
BstXI 1292 CCAGAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 591 ATCGAT AT^CGAT
CpoI 2195 CGGTCCG CG^G[AT]CCG
DsaI 657 CCATGG C^C[GA][CT]GG
EaeI 109 CGGCCA [CT]^GGCC[GA]
EcoRI 1329 GAATTC G^AATTC
HaeII 158 AGCGCC [GA]GCGC^[CT]
Hin1I 3 GGCGTC G[GA]^CG[CT]C
MamI 1903 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 488 CAATTG C^AATTG
MunI 488 CAATTG C^AATTG
NaeI 107 GCCGGC GCC^GGC
NcoI 657 CCATGG C^CATGG
NgoMIV 107 GCCGGC G^CCGGC
PflFI 1096 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1148 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1537 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 1922 TCATGA T^CATGA
RsrII 2195 CGGTCCG CG^G[AT]CCG
SapI 1227 GAAGAGC GCTCTTC(1/4)
ScaI 2006 AGTACT AGT^ACT
SspBI 1121 TGTACA T^GTACA
StuI 1304 AGGCCT AGG^CCT
Tth111I 1096 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1148 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 387 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflII 409 CTTAAG C^TTAAG
1746 CTTAAG
AocI 1579 CCTAAGG CC^T[ACGT]AGG
1756 CCTAAGG
ApaI 142 GGGCCC GGGCC^C
267 GGGCCC
AspHI 973 GAGCAC G[AT]GC[AT]^C
1803 GTGCAC
BfrI 409 CTTAAG C^TTAAG
1746 CTTAAG
BglI 619 GCCACTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2059 GCCTGGGAGGC
BglII 1013 AGATCT A^GATCT
1908 AGATCT
BsiHKAI 973 GAGCAC G[AT]GC[AT]^C
1803 GTGCAC
BsrFI 107 GCCGGC [GA]^CCGG[CT]
1972 ACCGGC
Bsu36I 1579 CCTAAGG CC^T[ACGT]AGG
1756 CCTAAGG
Cfr10I 107 GCCGGC [GA]^CCGG[CT]
1972 ACCGGC
Eco81I 1579 CCTAAGG CC^T[ACGT]AGG
1756 CCTAAGG
MaeII 43 ACGT A^CGT
1704 ACGT
MseI 410 TTAA T^TAA
1747 TTAA
MslI 2019 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2034 CACGGCAGTG
NspI 1508 ACATGC [GA]CATG^[CT]
2018 ACATGT
SmaI 2069 CCCGGG CCC^GGG
2175 CCCGGG
TaqI 534 TCGA T^CGA
592 TCGA
Tru9I 410 TTAA T^TAA
1747 TTAA
TthHB8I 534 TCGA T^CGA
592 TCGA
XcmI 359 CCAACGCCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2015 CCAACATGTCTGTGG
XmaCI 2069 CCCGGG C^CCGGG
2175 CCCGGG
XmaI 2069 CCCGGG C^CCGGG
2175 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
TseI Tsp45I Tsp509I TspRI XhoII