Restriction map for
pF1KSDA0222
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AclI AflII AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AvrII BamHI BciVI BclI BfrI
BlnI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI HindIII HpaI MfeI
MunI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI Psp1406I PvuI RcaI RsrII
ScaI SfuI SnaBI SpeI SspBI
SspI SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 3454 GGTACC G^GTACC
AccI 2214 GTCGAC GT^[AC][GT]AC
AcsI 2929 AAATTC [GA]^AATT[CT]
AflIII 1602 ACGCGT A^C[GA][CT]GT
AgeI 2745 ACCGGT A^CCGGT
Alw44I 577 GTGCAC G^TGCAC
ApaLI 577 GTGCAC G^TGCAC
ApoI 2929 AAATTC [GA]^AATT[CT]
Asp718I 3454 GGTACC G^GTACC
AspI 1231 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 158 TGCGCA TGC^GCA
BbrPI 574 CACGTG CAC^GTG
BbsI 1771 GAAGAC GAAGAC(2/6)
BcgI 1758 CGACGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1565 AGATCT A^GATCT
BpuAI 1771 GAAGAC GAAGAC(2/6)
BsaAI 574 CACGTG [CT]AC^GT[GA]
BsaBI 234 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2242 GAGACG CGTCTC(1/5)
BsmI 2476 GAATGC GAATGC(1/-1)
BstEII 2110 GGTCACC G^GT[ACGT]ACC
BstPI 2110 GGTCACC G^GT[ACGT]ACC
BstYI 1565 AGATCT [GA]^GATC[CT]
EcoO65I 2110 GGTCACC G^GT[ACGT]ACC
FseI 57 GGCCGGCC GGCCGG^CC
FspI 158 TGCGCA TGC^GCA
KpnI 3454 GGTACC GGTAC^C
KspI 1429 CCGCGG CCGC^GG
MamI 234 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1565 AGATCT [GA]^GATC[CT]
MluI 1602 ACGCGT A^CGCGT
MslI 2511 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 1231 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 69 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2745 ACCGGT A^CCGGT
PmaCI 574 CACGTG CAC^GTG
PmlI 574 CACGTG CAC^GTG
PshAI 1873 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1429 CCGCGG CCGC^GG
SalI 2214 GTCGAC G^TCGAC
SapI 2549 GCTCTTC GCTCTTC(1/4)
SbfI 2256 CCTGCAGG CCTGCA^GG
SexAI 778 ACCTGGT A^CC[AT]GGT
SgrAI 1914 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 2256 CCTGCAGG CCTGCA^GG
Tth111I 1231 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 69 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1981 TCTAGA T^CTAGA
XhoII 1565 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BbvCI 30 GCTGAGG CCTCAGC(-5/-2)
3027 GCTGAGG
BsaI 1871 GAGACC GGTCTC(1/5)
3437 GAGACC
EarI 2550 CTCTTC CTCTTC(1/4)
1840 GAAGAG
EcoT14I 509 CCAAGG C^C[AT][AT]GG
1925 CCAAGG
HincII 1237 GTCAAC GT[CT]^[GA]AC
2214 GTCGAC
HindII 1237 GTCAAC GT[CT]^[GA]AC
2214 GTCGAC
Ksp632I 2550 CTCTTC CTCTTC(1/4)
1840 GAAGAG
NheI 2581 GCTAGC G^CTAGC
2734 GCTAGC
PvuII 1258 CAGCTG CAG^CTG
2355 CAGCTG
SacI 138 GAGCTC GAGCT^C
1858 GAGCTC
SmlI 871 CTCAAG C^T[CT][GA]AG
1300 CTCAAG
SphI 490 GCATGC GCATG^C
2585 GCATGC
StyI 509 CCAAGG C^C[AT][AT]GG
1925 CCAAGG
Tsp509I 2416 AATT ^AATT
2930 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstAPI BstF5I BstNI
BstUI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MroI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
StuI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I XcmI XmaCI
XmaI