Restriction map for
pF1KSDA0230
##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AflII AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AviII
AvrII BbrPI BclI BfrI BlnI
BsaAI BsaBI BseRI BsiEI BsiWI
BsmBI BssHII Bst1107I BstBI BstZ17I
EagI EclXI Eco52I EcoRI EcoRV
FbaI FseI FspI HpaI KpnI
MamI MluI NdeI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshBI PvuI SbfI
ScaI SfuI SnaBI SpeI Sse8387I
SspI StuI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 3317 TCCGGA T^CCGGA
AclI 1834 AACGTT AA^CGTT
AhdI 2119 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 8 AGCGCT AGC^GCT
Asp700I 2316 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2119 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglII 201 AGATCT A^GATCT
BlpI 855 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 855 GCTGAGC GC^T[ACGT]AGC
BseAI 3317 TCCGGA T^CCGGA
BspDI 3340 ATCGAT AT^CGAT
BspEI 3317 TCCGGA T^CCGGA
BspLU11I 2201 ACATGT A^CATGT
BstXI 2492 CCACGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 855 GCTGAGC GC^T[ACGT]AGC
ClaI 3340 ATCGAT AT^CGAT
CpoI 2827 CGGTCCG CG^G[AT]CCG
DraI 333 TTTAAA TTT^AAA
Eam1105I 2119 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 8 AGCGCT AGC^GCT
MfeI 371 CAATTG C^AATTG
MroI 3317 TCCGGA T^CCGGA
MunI 371 CAATTG C^AATTG
NaeI 3677 GCCGGC GCC^GGC
NcoI 3434 CCATGG C^CATGG
NgoMIV 3677 GCCGGC G^CCGGC
PaeR7I 478 CTCGAG C^TCGAG
Psp1406I 1834 AACGTT AA^CGTT
RsrII 2827 CGGTCCG CG^G[AT]CCG
SacI 3412 GAGCTC GAGCT^C
SalI 2086 GTCGAC G^TCGAC
SfiI 75 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2176 CACCGGCG C[GA]^CCGG[CT]G
XbaI 405 TCTAGA T^CTAGA
XhoI 478 CTCGAG C^TCGAG
XmnI 2316 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 1843 GACGTC GACGT^C
3801 GACGTC
AccI 2086 GTCGAC GT^[AC][GT]AC
3523 GTCTAC
AcsI 330 AAATTT [GA]^AATT[CT]
1708 AAATTT
AocI 996 CCTCAGG CC^T[ACGT]AGG
1315 CCTCAGG
ApoI 330 AAATTT [GA]^AATT[CT]
1708 AAATTT
AspI 1255 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1321 GACAGAGTC
BbvCI 4109 CCTCAGC CCTCAGC(-5/-2)
252 GCTGAGG
BcgI 2152 GCAAACCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2624 GCATGTTCTTCG
BciVI 299 GGATAC GTATCC(6/5)
1986 GTATCC
BspHI 2579 TCATGA T^CATGA
2678 TCATGA
BsrBI 2610 GAGCGG CCGCTC(-3/-3)
3421 GAGCGG
BssSI 46 CTCGTG CACGAG(-5/-1)
3649 CTCGTG
BstAPI 150 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2543 GCAACGTGTGC
Bsu36I 996 CCTCAGG CC^T[ACGT]AGG
1315 CCTCAGG
DrdI 3514 GACTACAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4321 GACGGGCAGGTC
Eco81I 996 CCTCAGG CC^T[ACGT]AGG
1315 CCTCAGG
EcoNI 2505 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3114 CCTGGGGGAGG
HindIII 3299 AAGCTT A^AGCTT
4346 AAGCTT
PflFI 1255 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1321 GACAGAGTC
RcaI 2579 TCATGA T^CATGA
2678 TCATGA
SapI 715 GAAGAGC GCTCTTC(1/4)
1291 GCTCTTC
Tth111I 1255 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1321 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI SmaI
SmlI SphI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI