Restriction map for
pF1KSDA0237
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI HgaI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 875 GGCGCC G[GA]^CG[CT]C
Alw44I 478 GTGCAC G^TGCAC
ApaLI 478 GTGCAC G^TGCAC
AvaI 533 CTCGGG C^[CT]CG[GA]G
AviII 236 TGCGCA TGC^GCA
BanII 57 GAGCTC G[GA]GC[CT]^C
BbeI 875 GGCGCC GGCGC^C
BbvCI 795 CCTCAGC CCTCAGC(-5/-2)
BfaI 382 CTAG C^TAG
BglI 610 GCCTGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 875 GGCGCC G[GA]^CG[CT]C
BsaWI 497 ACCGGA [AT]^CCGG[AT]
BseRI 837 CTCCTC GAGGAG(10/8)
BsoBI 533 CTCGGG C^[CT]CG[GA]G
BsrDI 483 CATTGC GCAATG(2/0)
BsrFI 811 ACCGGC [GA]^CCGG[CT]
BstAPI 669 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 807 GGTCACC G^GT[ACGT]ACC
BstPI 807 GGTCACC G^GT[ACGT]ACC
Cfr10I 811 ACCGGC [GA]^CCGG[CT]
EarI 826 CTCTTC CTCTTC(1/4)
EcoO65I 807 GGTCACC G^GT[ACGT]ACC
EcoT14I 467 CCATGG C^C[AT][AT]GG
FauI 104 GCGGG CCCGC(4/6)
FspI 236 TGCGCA TGC^GCA
Hin1I 875 GGCGCC G[GA]^CG[CT]C
KasI 875 GGCGCC G^GCGCC
Ksp632I 826 CTCTTC CTCTTC(1/4)
KspI 803 CCGCGG CCGC^GG
MaeI 382 CTAG C^TAG
MaeII 358 ACGT A^CGT
MseI 5 TTAA T^TAA
NarI 875 GGCGCC GG^CGCC
NcoI 467 CCATGG C^CATGG
PstI 709 CTGCAG CTGCA^G
PvuII 508 CAGCTG CAG^CTG
SacI 57 GAGCTC GAGCT^C
SacII 803 CCGCGG CCGC^GG
SfcI 709 CTGCAG C^T[GA][CT]AG
SfiI 609 GGCCTGCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 875 GGCGCC GGC^GCC
StyI 467 CCATGG C^C[AT][AT]GG
Tru9I 5 TTAA T^TAA
XcmI 833 CCACCTCCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 273 CGCG CG^CG
804 CGCG
AfaI 663 GTAC GT^AC
819 GTAC
BamHI 85 GGATCC G^GATCC
862 GGATCC
BanI 148 GGTGCC G^G[CT][GA]CC
875 GGCGCC
BsmAI 262 GAGAC GTCTC(1/5)
728 GAGAC
BspMI 593 ACCTGC ACCTGC(4/8)
711 GCAGGT
BstF5I 246 CATCC GGATG(2/0)
414 GGATG
BstUI 273 CGCG CG^CG
804 CGCG
BstXI 41 CCAGGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
152 CCAAGATGGTGG
BstYI 85 GGATCC [GA]^GATC[CT]
862 GGATCC
DsaI 467 CCATGG C^C[GA][CT]GG
803 CCGCGG
FokI 246 CATCC GGATG(9/13)
414 GGATG
HaeII 799 AGCGCC [GA]GCGC^[CT]
875 GGCGCC
MaeIII 295 GTCAC ^GT[ACGT]AC
808 GTCAC
MflI 85 GGATCC [GA]^GATC[CT]
862 GGATCC
MslI 270 CATCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
759 CATGGGCATG
MspA1I 508 CAGCTG C[AC]G^C[GT]G
803 CCGCGG
MvnI 273 CGCG CG^CG
804 CGCG
PpuMI 18 AGGTCCT [GA]G^G[AT]CC[CT]
690 GGGACCC
RsaI 663 GTAC GT^AC
819 GTAC
SfaNI 282 GATGC GCATC(5/9)
269 GCATC
Tsp45I 295 GTCAC ^GT[GC]AC
808 GTCAC
XhoII 85 GGATCC [GA]^GATC[CT]
862 GGATCC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BalI BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MboII MluNI MnlI MscI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI TseI TspRI