Restriction map for
pF1KSDA0238
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII AccI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApaI AscI
AseI AsnI AspEI AspI AviII
AvrII BbrPI BbvCI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CelII CpoI
DraII DraIII DrdI Eam1105I Eco47III
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StyI Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 296 GGTACC G^GTACC
AccII 1369 CGCG CG^CG
AclI 468 AACGTT AA^CGTT
AcyI 124 GGCGCC G[GA]^CG[CT]C
Alw44I 1202 GTGCAC G^TGCAC
AocI 1896 CCTCAGG CC^T[ACGT]AGG
ApaLI 1202 GTGCAC G^TGCAC
Asp700I 280 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 296 GGTACC G^GTACC
AvaI 527 CTCGAG C^[CT]CG[GA]G
BalI 953 TGGCCA TGG^CCA
BbeI 124 GGCGCC GGCGC^C
BcgI 350 CGATCGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1490 TGATCA T^GATCA
BfaI 835 CTAG C^TAG
BglI 689 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 124 GGCGCC G[GA]^CG[CT]C
BsaI 220 GAGACC GGTCTC(1/5)
BseRI 691 CTCCTC GAGGAG(10/8)
BsgI 1473 CTGCAC GTGCAG(16/14)
BsmAI 220 GAGAC GTCTC(1/5)
BsoBI 527 CTCGAG C^[CT]CG[GA]G
BspDI 1636 ATCGAT AT^CGAT
BsrFI 1132 GCCGGT [GA]^CCGG[CT]
BstAPI 1056 GCAAGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 482 GGTTACC G^GT[ACGT]ACC
BstPI 482 GGTTACC G^GT[ACGT]ACC
BstUI 1369 CGCG CG^CG
Bsu36I 1896 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1132 GCCGGT [GA]^CCGG[CT]
ClaI 1636 ATCGAT AT^CGAT
DraI 1530 TTTAAA TTT^AAA
EagI 1130 CGGCCG C^GGCCG
EclXI 1130 CGGCCG C^GGCCG
Eco52I 1130 CGGCCG C^GGCCG
Eco81I 1896 CCTCAGG CC^T[ACGT]AGG
EcoNI 489 CCTGTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 482 GGTTACC G^GT[ACGT]ACC
FauI 784 GCGGG CCCGC(4/6)
FbaI 1490 TGATCA T^GATCA
HgaI 1438 GCGTC GACGC(5/10)
Hin1I 124 GGCGCC G[GA]^CG[CT]C
KasI 124 GGCGCC G^GCGCC
KpnI 296 GGTACC GGTAC^C
MaeI 835 CTAG C^TAG
MluNI 953 TGGCCA TGG^CCA
MscI 953 TGGCCA TGG^CCA
MvnI 1369 CGCG CG^CG
NarI 124 GGCGCC GG^CGCC
PaeR7I 527 CTCGAG C^TCGAG
PshAI 996 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 468 AACGTT AA^CGTT
PstI 1516 CTGCAG CTGCA^G
PvuI 350 CGATCG CGAT^CG
SapI 1902 GAAGAGC GCTCTTC(1/4)
SfiI 688 GGCCTCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 124 GGCGCC GGC^GCC
SmlI 527 CTCGAG C^T[CT][GA]AG
SphI 1145 GCATGC GCATG^C
SspI 828 AATATT AAT^ATT
SwaI 1529 ATTTAAAT ATTT^AAAT
XhoI 527 CTCGAG C^TCGAG
XmnI 280 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccIII 1446 TCCGGA T^CCGGA
1742 TCCGGA
BamHI 632 GGATCC G^GATCC
1449 GGATCC
BanI 124 GGCGCC G^G[CT][GA]CC
296 GGTACC
BciVI 624 GTATCC GTATCC(6/5)
964 GTATCC
BpmI 132 CTCCAG CTGGAG(16/14)
1724 CTCCAG
BseAI 1446 TCCGGA T^CCGGA
1742 TCCGGA
BsiEI 350 CGATCG CG[GA][CT]^CG
1130 CGGCCG
BspEI 1446 TCCGGA T^CCGGA
1742 TCCGGA
BspMI 1514 ACCTGC ACCTGC(4/8)
479 GCAGGT
BsrDI 195 CATTGC GCAATG(2/0)
813 CATTGC
CfoI 125 GCGC GCG^C
1427 GCGC
EaeI 953 TGGCCA [CT]^GGCC[GA]
1130 CGGCCG
EarI 101 CTCTTC CTCTTC(1/4)
1902 GAAGAG
HaeII 124 GGCGCC [GA]GCGC^[CT]
1426 AGCGCC
HhaI 125 GCGC GCG^C
1427 GCGC
HinP1I 125 GCGC G^CGC
1427 GCGC
Ksp632I 101 CTCTTC CTCTTC(1/4)
1902 GAAGAG
MaeII 469 ACGT A^CGT
1652 ACGT
MroI 1446 TCCGGA T^CCGGA
1742 TCCGGA
NspI 334 ACATGC [GA]CATG^[CT]
1145 GCATGC
PvuII 925 CAGCTG CAG^CTG
1496 CAGCTG
SacI 163 GAGCTC GAGCT^C
207 GAGCTC
StuI 1266 AGGCCT AGG^CCT
1893 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BcnI BmyI BpuAI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII