Restriction map for
pF1KSDA0252
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# NO CUT ENZYMES ( 124 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BcgI BciVI BfrI
BlnI BsaAI BsaWI BseAI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 168 GACGTC GACGT^C
AccI 811 GTCTAC GT^[AC][GT]AC
AcyI 168 GACGTC G[GA]^CG[CT]C
AfaI 1861 GTAC GT^AC
AflIII 1182 ACGCGT A^C[GA][CT]GT
ApaI 458 GGGCCC GGGCC^C
BamHI 1653 GGATCC G^GATCC
BanI 1135 GGCACC G^G[CT][GA]CC
BbvCI 1447 GCTGAGG CCTCAGC(-5/-2)
BclI 1344 TGATCA T^GATCA
BsaBI 1044 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 168 GACGTC G[GA]^CG[CT]C
BsaI 1703 GAGACC GGTCTC(1/5)
BsmBI 166 GAGACG CGTCTC(1/5)
BsmFI 87 GTCCC GGGAC(10/14)
BsmI 1740 GAATGC GAATGC(1/-1)
BsrBI 867 CCGCTC CCGCTC(-3/-3)
BsrDI 1172 GCAATG GCAATG(2/0)
BstXI 1125 CCAACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 105 GCGC GCG^C
FauI 690 GCGGG CCCGC(4/6)
FbaI 1344 TGATCA T^GATCA
HgaI 23 GCGTC GACGC(5/10)
HhaI 105 GCGC GCG^C
Hin1I 168 GACGTC G[GA]^CG[CT]C
HinP1I 105 GCGC G^CGC
MaeII 169 ACGT A^CGT
MamI 1044 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1182 ACGCGT A^CGCGT
MslI 1168 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 1644 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1451 AGGACCT [GA]G^G[AT]CC[CT]
PstI 146 CTGCAG CTGCA^G
RsaI 1861 GTAC GT^AC
SfcI 146 CTGCAG C^T[GA][CT]AG
SmlI 716 CTCAAG C^T[CT][GA]AG
SphI 1268 GCATGC GCATG^C
Van91I 1644 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1284 TCTAGA T^CTAGA
XcmI 414 CCATGATGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 22 CGCG CG^CG
1183 CGCG
AocI 284 CCTCAGG CC^T[ACGT]AGG
328 CCTGAGG
BcnI 1080 CCGGG CC^[GC]GG
1581 CCGGG
BglI 1612 GCCCTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1723 GCCATCTTGGC
BglII 1848 AGATCT A^GATCT
1966 AGATCT
BlpI 320 GCTCAGC GC^T[ACGT]AGC
769 GCTGAGC
BpmI 1504 CTGGAG CTGGAG(16/14)
1757 CTGGAG
Bpu1102I 320 GCTCAGC GC^T[ACGT]AGC
769 GCTGAGC
BstUI 22 CGCG CG^CG
1183 CGCG
Bsu36I 284 CCTCAGG CC^T[ACGT]AGG
328 CCTGAGG
CelII 320 GCTCAGC GC^T[ACGT]AGC
769 GCTGAGC
DsaI 722 CCATGG C^C[GA][CT]GG
1442 CCATGG
Eco81I 284 CCTCAGG CC^T[ACGT]AGG
328 CCTGAGG
EcoNI 69 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
328 CCTGAGGAAGG
EcoRI 1216 GAATTC G^AATTC
1818 GAATTC
HapII 1080 CCGG C^CGG
1581 CCGG
HpaII 1080 CCGG C^CGG
1581 CCGG
MspI 1080 CCGG C^CGG
1581 CCGG
MvnI 22 CGCG CG^CG
1183 CGCG
NciI 1080 CCGGG CC^[GC]GG
1581 CCGGG
NcoI 722 CCATGG C^CATGG
1442 CCATGG
NspI 1001 ACATGC [GA]CATG^[CT]
1268 GCATGC
SfaNI 1771 GATGC GCATC(5/9)
1049 GCATC
TaqI 32 TCGA T^CGA
966 TCGA
TthHB8I 32 TCGA T^CGA
966 TCGA
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII SacI Sau3AI Sau96I
ScrFI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII