Restriction map for
pF1KSDA0262
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BanI BbeI BbrPI BciVI BclI
BfrI BglI BlnI BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
FbaI FseI Hin1I HindIII KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NarI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 943 GTAGAC GT^[AC][GT]AC
AocI 1378 CCTGAGG CC^T[ACGT]AGG
ApaI 1994 GGGCCC GGGCC^C
AspI 1673 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 644 TGCGCA TGC^GCA
BalI 453 TGGCCA TGG^CCA
BamHI 175 GGATCC G^GATCC
BcgI 2411 GCACCTCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1026 CCGGG CC^[GC]GG
BglII 1619 AGATCT A^GATCT
BlpI 6 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 6 GCTGAGC GC^T[ACGT]AGC
BsaBI 1420 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1752 GGTCTC GGTCTC(1/5)
BsmAI 1753 GTCTC GTCTC(1/5)
BspHI 2318 TCATGA T^CATGA
BsrBI 111 CCGCTC CCGCTC(-3/-3)
BsrFI 133 GCCGGC [GA]^CCGG[CT]
BssSI 1579 CTCGTG CACGAG(-5/-1)
BstAPI 1014 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 390 CCAGTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1378 CCTGAGG CC^T[ACGT]AGG
CelII 6 GCTGAGC GC^T[ACGT]AGC
Cfr10I 133 GCCGGC [GA]^CCGG[CT]
DrdI 2052 GACCCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1378 CCTGAGG CC^T[ACGT]AGG
EcoRV 1685 GATATC GAT^ATC
EcoT22I 741 ATGCAT ATGCA^T
FspI 644 TGCGCA TGC^GCA
HaeII 1808 AGCGCC [GA]GCGC^[CT]
HgaI 1677 GCGTC GACGC(5/10)
HincII 619 GTTAAC GT[CT]^[GA]AC
HindII 619 GTTAAC GT[CT]^[GA]AC
HpaI 619 GTTAAC GTT^AAC
MamI 1420 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 453 TGGCCA TGG^CCA
MscI 453 TGGCCA TGG^CCA
NaeI 133 GCCGGC GCC^GGC
NciI 1026 CCGGG CC^[GC]GG
NcoI 1989 CCATGG C^CATGG
NdeI 1546 CATATG CA^TATG
NgoMIV 133 GCCGGC G^CCGGC
NsiI 741 ATGCAT ATGCA^T
NspI 739 GCATGC [GA]CATG^[CT]
PaeR7I 1906 CTCGAG C^TCGAG
PflFI 1673 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 558 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1968 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 698 CTGCAG CTGCA^G
RcaI 2318 TCATGA T^CATGA
SapI 323 GCTCTTC GCTCTTC(1/4)
ScaI 983 AGTACT AGT^ACT
SphI 739 GCATGC GCATG^C
StuI 1394 AGGCCT AGG^CCT
Tth111I 1673 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 558 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1321 TCTAGA T^CTAGA
XhoI 1906 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 432 GAATTT [GA]^AATT[CT]
2286 AAATTC
ApoI 432 GAATTT [GA]^AATT[CT]
2286 AAATTC
AspHI 9 GAGCTC G[AT]GC[AT]^C
1120 GAGCTC
BbvCI 1497 CCTCAGC CCTCAGC(-5/-2)
2145 GCTGAGG
BsiHKAI 9 GAGCTC G[AT]GC[AT]^C
1120 GAGCTC
BspMI 1945 ACCTGC ACCTGC(4/8)
1014 GCAGGT
DraIII 761 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1558 CACCCTGTG
EaeI 453 TGGCCA [CT]^GGCC[GA]
1154 TGGCCG
EcoT14I 1220 CCTTGG C^C[AT][AT]GG
1989 CCATGG
MslI 850 CATCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
873 CATTACGATG
PvuII 883 CAGCTG CAG^CTG
1063 CAGCTG
SacI 9 GAGCTC GAGCT^C
1120 GAGCTC
SfcI 698 CTGCAG C^T[GA][CT]AG
1915 CTACAG
StyI 1220 CCTTGG C^C[AT][AT]GG
1989 CCATGG
TfiI 1911 GAATC G^A[AT]TC
1957 GATTC
XcmI 989 CCAAGTTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1527 CCAAGCGGAAGATGG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII