Restriction map for
pF1KSDA0269
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BcnI BfrI BglI BglII BpuAI
BsaHI BsaI BsaWI BseAI BsgI
BsoBI Bsp1407I BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I CpoI DraI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HapII HgaI Hin1I
HpaI HpaII KasI KpnI KspI
MluI MluNI MroI MscI MspA1I
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcsI 1651 GAATTT [GA]^AATT[CT]
AflIII 213 ACGTGT A^C[GA][CT]GT
ApoI 1651 GAATTT [GA]^AATT[CT]
AseI 1404 ATTAAT AT^TAAT
AsnI 1404 ATTAAT AT^TAAT
Asp700I 294 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 584 GAGCAC G[AT]GC[AT]^C
AvrII 28 CCTAGG C^CTAGG
BanII 960 GAGCCC G[GA]GC[CT]^C
BbvCI 799 GCTGAGG CCTCAGC(-5/-2)
BlnI 28 CCTAGG C^CTAGG
BlpI 1564 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1564 GCTAAGC GC^T[ACGT]AGC
BsaAI 724 TACGTG [CT]AC^GT[GA]
BsaBI 649 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 336 CGATCG CG[GA][CT]^CG
BsiHKAI 584 GAGCAC G[AT]GC[AT]^C
BsiWI 371 CGTACG C^GTACG
BsmBI 367 GAGACG CGTCTC(1/5)
BsmFI 1391 GTCCC GGGAC(10/14)
BsmI 1307 GCATTC GAATGC(1/-1)
BspDI 22 ATCGAT AT^CGAT
BspMI 1437 ACCTGC ACCTGC(4/8)
BsrDI 203 CATTGC GCAATG(2/0)
BstAPI 859 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 742 GGATCT [GA]^GATC[CT]
CelII 1564 GCTAAGC GC^T[ACGT]AGC
CfoI 1455 GCGC GCG^C
ClaI 22 ATCGAT AT^CGAT
DraII 1389 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1247 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1389 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 850 ATGCAT ATGCA^T
HaeII 1454 AGCGCC [GA]GCGC^[CT]
HhaI 1455 GCGC GCG^C
HinP1I 1455 GCGC G^CGC
HindIII 296 AAGCTT A^AGCTT
MamI 649 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1163 CAATTG C^AATTG
MflI 742 GGATCT [GA]^GATC[CT]
MslI 733 CATATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1163 CAATTG C^AATTG
NdeI 733 CATATG CA^TATG
NlaIV 1390 GGTCCC GG[ACGT]^[ACGT]CC
NsiI 850 ATGCAT ATGCA^T
PflMI 847 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 194 GAGTC GAGTC(4/5)
PpuMI 1389 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1404 ATTAAT AT^TAAT
PvuI 336 CGATCG CGAT^CG
SapI 1543 GAAGAGC GCTCTTC(1/4)
SspI 91 AATATT AAT^ATT
Van91I 847 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 549 TCTAGA T^CTAGA
XcmI 89 CCAATATTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 742 GGATCT [GA]^GATC[CT]
XmnI 294 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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BmyI 584 GAGCAC G[AGT]GC[ACT]^C
960 GAGCCC
Bsp1286I 584 GAGCAC G[AGT]GC[ACT]^C
960 GAGCCC
BspHI 561 TCATGA T^CATGA
591 TCATGA
HaeIII 621 GGCC GG^CC
688 GGCC
HincII 196 GTCAAC GT[CT]^[GA]AC
1005 GTCAAC
HindII 196 GTCAAC GT[CT]^[GA]AC
1005 GTCAAC
RcaI 561 TCATGA T^CATGA
591 TCATGA
SfcI 899 CTACAG C^T[GA][CT]AG
935 CTACAG
TfiI 1633 GATTC G^A[AT]TC
1645 GATTC
Tsp45I 927 GTCAC ^GT[GC]AC
1327 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BfaI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I