Restriction map for
pF1KSDA0275
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI MaeI
MaeII MamI MfeI MluI MroI
MseI MslI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 73 GACGCC G[GA]^CG[CT]C
AgeI 598 ACCGGT A^CCGGT
AlwNI 604 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 531 GGGCCC GGGCC^C
Asp700I 174 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 352 GAGCAC G[AT]GC[AT]^C
BalI 453 TGGCCA TGG^CCA
BciVI 249 GGATAC GTATCC(6/5)
BglI 694 GCCGGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 677 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 677 GCTCAGC GC^T[ACGT]AGC
BsaHI 73 GACGCC G[GA]^CG[CT]C
BsgI 282 GTGCAG GTGCAG(16/14)
BsiEI 371 CGACCG CG[GA][CT]^CG
BsiHKAI 352 GAGCAC G[AT]GC[AT]^C
BspHI 654 TCATGA T^CATGA
BssHII 3 GCGCGC G^CGCGC
BstAPI 407 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 962 AGATCC [GA]^GATC[CT]
CelII 677 GCTCAGC GC^T[ACGT]AGC
DraIII 292 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 787 CTCTTC CTCTTC(1/4)
HgaI 73 GACGC GACGC(5/10)
Hin1I 73 GACGCC G[GA]^CG[CT]C
Ksp632I 787 CTCTTC CTCTTC(1/4)
KspI 50 CCGCGG CCGC^GG
MflI 962 AGATCC [GA]^GATC[CT]
MluNI 453 TGGCCA TGG^CCA
MscI 453 TGGCCA TGG^CCA
NaeI 694 GCCGGC GCC^GGC
NcoI 384 CCATGG C^CATGG
NgoMIV 694 GCCGGC G^CCGGC
PflMI 746 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 598 ACCGGT A^CCGGT
RcaI 654 TCATGA T^CATGA
SacII 50 CCGCGG CCGC^GG
SmaI 9 CCCGGG CCC^GGG
SmlI 85 CTCAAG C^T[CT][GA]AG
Tsp509I 109 AATT ^AATT
Van91I 746 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 962 AGATCC [GA]^GATC[CT]
XmaCI 9 CCCGGG C^CCGGG
XmaI 9 CCCGGG C^CCGGG
XmnI 174 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 150 GTAC GT^AC
831 GTAC
AlwI 963 GATCC GGATC(4/5)
359 GGATC
BsaI 97 GAGACC GGTCTC(1/5)
888 GGTCTC
BsaWI 598 ACCGGT [AT]^CCGG[AT]
1028 ACCGGA
BsmBI 185 GAGACG CGTCTC(1/5)
1195 GAGACG
BsrDI 303 CATTGC GCAATG(2/0)
126 GCAATG
BsrFI 598 ACCGGT [GA]^CCGG[CT]
694 GCCGGC
BstXI 704 CCAGCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
815 CCATCAACCTGG
Cfr10I 598 ACCGGT [GA]^CCGG[CT]
694 GCCGGC
HinfI 397 GACTC G^A[ACGT]TC
742 GACTC
HphI 276 GGTGA GGTGA(8/7)
1057 GGTGA
PleI 397 GACTC GAGTC(4/5)
742 GACTC
PpuMI 260 AGGACCC [GA]G^G[AT]CC[CT]
605 AGGACCT
RsaI 150 GTAC GT^AC
831 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI