Restriction map for
pF1KSDA0283
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# NO CUT ENZYMES ( 71 enzymes) #
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Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BcgI BclI BfrI BlnI
BsiEI BsiWI Bsp1407I BspLU11I BsrFI
BsrGI BssHII BssSI BstBI Cfr10I
CpoI DraI EagI EclXI Eco52I
EcoNI EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 2476 GACGTC GACGT^C
AccIII 1634 TCCGGA T^CCGGA
AflIII 4214 ACGTGT A^C[GA][CT]GT
AhdI 2877 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3406 GTGCAC G^TGCAC
AocI 36 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4049 AGCGCT AGC^GCT
ApaLI 3406 GTGCAC G^TGCAC
AspEI 2877 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 538 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 64 GGCGCC GGCGC^C
BbrPI 996 CACGTG CAC^GTG
BbvCI 24 CCTCAGC CCTCAGC(-5/-2)
BciVI 2848 GGATAC GTATCC(6/5)
BglII 1502 AGATCT A^GATCT
BlpI 2430 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2430 GCTCAGC GC^T[ACGT]AGC
BseAI 1634 TCCGGA T^CCGGA
BspDI 2747 ATCGAT AT^CGAT
BspEI 1634 TCCGGA T^CCGGA
Bst1107I 4288 GTATAC GTA^TAC
BstAPI 763 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4288 GTATAC GTA^TAC
Bsu36I 36 CCTGAGG CC^T[ACGT]AGG
CelII 2430 GCTCAGC GC^T[ACGT]AGC
ClaI 2747 ATCGAT AT^CGAT
Eam1105I 2877 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4049 AGCGCT AGC^GCT
Eco81I 36 CCTGAGG CC^T[ACGT]AGG
EcoRI 2190 GAATTC G^AATTC
HgaI 782 GCGTC GACGC(5/10)
HindIII 670 AAGCTT A^AGCTT
KasI 64 GGCGCC G^GCGCC
KspI 2502 CCGCGG CCGC^GG
MroI 1634 TCCGGA T^CCGGA
NarI 64 GGCGCC GG^CGCC
PaeR7I 1605 CTCGAG C^TCGAG
PflFI 538 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 996 CACGTG CAC^GTG
PmlI 996 CACGTG CAC^GTG
PpuMI 548 GGGTCCT [GA]G^G[AT]CC[CT]
SacII 2502 CCGCGG CCGC^GG
SalI 1018 GTCGAC G^TCGAC
SfoI 64 GGCGCC GGC^GCC
Tth111I 538 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1467 TCTAGA T^CTAGA
XhoI 1605 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 64 GGCGCC G[GA]^CG[CT]C
2476 GACGTC
Asp700I 1444 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1620 GAAAGTGTTC
BbsI 1444 GAAGAC GAAGAC(2/6)
3870 GAAGAC
BglI 3182 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3431 GCCTCTGTGGC
BpuAI 1444 GAAGAC GAAGAC(2/6)
3870 GAAGAC
BsaAI 367 TACGTG [CT]AC^GT[GA]
996 CACGTG
BsaHI 64 GGCGCC G[GA]^CG[CT]C
2476 GACGTC
BsaWI 1155 TCCGGT [AT]^CCGG[AT]
1634 TCCGGA
BsmBI 501 CGTCTC CGTCTC(1/5)
3909 CGTCTC
BsmI 621 GAATGC GAATGC(1/-1)
1959 GAATGC
BspHI 221 TCATGA T^CATGA
1364 TCATGA
BspMI 2015 ACCTGC ACCTGC(4/8)
2217 GCAGGT
BsrBI 807 CCGCTC CCGCTC(-3/-3)
1838 CCGCTC
DraIII 3557 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
4222 CACATCGTG
DrdI 3010 GACACGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3028 GACATTAAAGTC
Hin1I 64 GGCGCC G[GA]^CG[CT]C
2476 GACGTC
PflMI 594 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3973 CCACAGGATGG
RcaI 221 TCATGA T^CATGA
1364 TCATGA
SmlI 1605 CTCGAG C^T[CT][GA]AG
2879 CTCAAG
SspI 3945 AATATT AAT^ATT
4307 AATATT
StuI 1350 AGGCCT AGG^CCT
2681 AGGCCT
Van91I 594 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3973 CCACAGGATGG
XmnI 1444 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1620 GAAAGTGTTC
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI