Restriction map for
pF1KSDA0287
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccIII AflII AflIII Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BfrI
BglII BlnI BsaBI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoRV FseI FspI
HpaI KasI KpnI MamI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI NspI NspV PacI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 4327 GACGTC GACGT^C
AclI 1136 AACGTT AA^CGTT
AgeI 635 ACCGGT A^CCGGT
AlwNI 3719 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2011 GGGCCC GGGCC^C
Asp700I 4311 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2519 CTCGAG C^[CT]CG[GA]G
BalI 420 TGGCCA TGG^CCA
BanI 4291 GGTGCC G^G[CT][GA]CC
BclI 4217 TGATCA T^GATCA
BglI 1180 GCCGCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3526 CACGTA [CT]AC^GT[GA]
BsiEI 633 CGACCG CG[GA][CT]^CG
BsoBI 2519 CTCGAG C^[CT]CG[GA]G
BspHI 2598 TCATGA T^CATGA
BspMI 3994 GCAGGT ACCTGC(4/8)
BstEII 106 GGTGACC G^GT[ACGT]ACC
BstPI 106 GGTGACC G^GT[ACGT]ACC
EcoO65I 106 GGTGACC G^GT[ACGT]ACC
EcoRI 2389 GAATTC G^AATTC
FbaI 4217 TGATCA T^GATCA
HaeII 803 GGCGCT [GA]GCGC^[CT]
HindIII 234 AAGCTT A^AGCTT
KspI 199 CCGCGG CCGC^GG
MluNI 420 TGGCCA TGG^CCA
MscI 420 TGGCCA TGG^CCA
NcoI 2862 CCATGG C^CATGG
PaeR7I 2519 CTCGAG C^TCGAG
PinAI 635 ACCGGT A^CCGGT
PpuMI 3562 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1136 AACGTT AA^CGTT
RcaI 2598 TCATGA T^CATGA
SacI 1723 GAGCTC GAGCT^C
SacII 199 CCGCGG CCGC^GG
ScaI 4313 AGTACT AGT^ACT
SgrAI 526 CACCGGCG C[GA]^CCGG[CT]G
XhoI 2519 CTCGAG C^TCGAG
XmnI 4311 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 1930 GTCTAC GT^[AC][GT]AC
2040 GTCTAC
AcyI 1649 GGCGTC G[GA]^CG[CT]C
4327 GACGTC
AspI 2305 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2884 GACCCTGTC
BsaHI 1649 GGCGTC G[GA]^CG[CT]C
4327 GACGTC
BsaWI 122 ACCGGA [AT]^CCGG[AT]
635 ACCGGT
BsrDI 973 GCAATG GCAATG(2/0)
2465 GCAATG
BsrFI 527 ACCGGC [GA]^CCGG[CT]
635 ACCGGT
BssSI 152 CACGAG CACGAG(-5/-1)
2432 CTCGTG
Cfr10I 527 ACCGGC [GA]^CCGG[CT]
635 ACCGGT
DraII 2010 AGGGCCC [GA]G^G[ACGT]CC[CT]
3562 GGGTCCT
DraIII 559 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1090 CACAGTGTG
EcoNI 324 CCTCGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2269 CCTTGTGAAGG
EcoO109I 2010 AGGGCCC [GA]G^G[ACGT]CC[CT]
3562 GGGTCCT
EcoT14I 1184 CCTTGG C^C[AT][AT]GG
2862 CCATGG
EcoT22I 734 ATGCAT ATGCA^T
1045 ATGCAT
HgaI 435 GACGC GACGC(5/10)
1650 GCGTC
Hin1I 1649 GGCGTC G[GA]^CG[CT]C
4327 GACGTC
HincII 3031 GTTGAC GT[CT]^[GA]AC
3502 GTCAAC
HindII 3031 GTTGAC GT[CT]^[GA]AC
3502 GTCAAC
MfeI 959 CAATTG C^AATTG
2876 CAATTG
MunI 959 CAATTG C^AATTG
2876 CAATTG
NsiI 734 ATGCAT ATGCA^T
1045 ATGCAT
PflFI 2305 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2884 GACCCTGTC
PvuII 3109 CAGCTG CAG^CTG
3722 CAGCTG
SmlI 2519 CTCGAG C^T[CT][GA]AG
2816 CTCAAG
StyI 1184 CCTTGG C^C[AT][AT]GG
2862 CCATGG
Tth111I 2305 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2884 GACCCTGTC
XcmI 93 CCATTCTTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
151 CCACGAGACCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI AlwI ApoI AspEI AspHI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DsaI EaeI Eam1105I EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV PflMI PleI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII