Restriction map for
pF1KSDA0289
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AvrII BbrPI BcgI BfrI BlnI
BsaAI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BstBI ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoNI EcoT22I FseI
HpaI KspI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SfiI SfuI
SnaBI SphI SspBI StuI SwaI
Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 522 GTATAC GT^[AC][GT]AC
AcyI 2004 GGCGCC G[GA]^CG[CT]C
AspI 1898 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 222 CCCGGG C^[CT]CG[GA]G
BbeI 2004 GGCGCC GGCGC^C
BciVI 3845 GTATCC GTATCC(6/5)
BglI 1663 GCCGAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1164 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2004 GGCGCC G[GA]^CG[CT]C
BsaWI 2432 TCCGGT [AT]^CCGG[AT]
BsiEI 65 CGGCCG CG[GA][CT]^CG
BsoBI 222 CCCGGG C^[CT]CG[GA]G
BspLU11I 3359 ACATGT A^CATGT
BspMI 2625 GCAGGT ACCTGC(4/8)
BsrBI 2698 GAGCGG CCGCTC(-3/-3)
Bst1107I 522 GTATAC GTA^TAC
BstEII 2886 GGTGACC G^GT[ACGT]ACC
BstPI 2886 GGTGACC G^GT[ACGT]ACC
BstZ17I 522 GTATAC GTA^TAC
DraIII 3366 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 65 CGGCCG C^GGCCG
EclXI 65 CGGCCG C^GGCCG
Eco52I 65 CGGCCG C^GGCCG
EcoO65I 2886 GGTGACC G^GT[ACGT]ACC
EcoRI 3610 GAATTC G^AATTC
EcoRV 304 GATATC GAT^ATC
HgaI 2087 GACGC GACGC(5/10)
Hin1I 2004 GGCGCC G[GA]^CG[CT]C
HincII 1210 GTCAAC GT[CT]^[GA]AC
HindII 1210 GTCAAC GT[CT]^[GA]AC
KasI 2004 GGCGCC G^GCGCC
MamI 1164 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2004 GGCGCC GG^CGCC
PflFI 1898 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3001 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 822 CCTGCAGG CCTGCA^GG
SexAI 1559 ACCTGGT A^CC[AT]GGT
SfoI 2004 GGCGCC GGC^GCC
SmaI 222 CCCGGG CCC^GGG
Sse8387I 822 CCTGCAGG CCTGCA^GG
Tth111I 1898 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 222 CCCGGG C^CCGGG
XmaI 222 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Acc65I 2405 GGTACC G^GTACC
2612 GGTACC
AcsI 1839 GAATTT [GA]^AATT[CT]
3610 GAATTC
AflIII 3359 ACATGT A^C[GA][CT]GT
3823 ACGTGT
AocI 2713 CCTAAGG CC^T[ACGT]AGG
3813 CCTCAGG
ApoI 1839 GAATTT [GA]^AATT[CT]
3610 GAATTC
Asp718I 2405 GGTACC G^GTACC
2612 GGTACC
AviII 209 TGCGCA TGC^GCA
650 TGCGCA
BclI 1163 TGATCA T^GATCA
3326 TGATCA
BsmI 1441 GAATGC GAATGC(1/-1)
1493 GCATTC
BstAPI 2789 GCATGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3686 GCAGTTGGTGC
Bsu36I 2713 CCTAAGG CC^T[ACGT]AGG
3813 CCTCAGG
Eco81I 2713 CCTAAGG CC^T[ACGT]AGG
3813 CCTCAGG
FbaI 1163 TGATCA T^GATCA
3326 TGATCA
FspI 209 TGCGCA TGC^GCA
650 TGCGCA
HindIII 2071 AAGCTT A^AGCTT
3723 AAGCTT
KpnI 2405 GGTACC GGTAC^C
2612 GGTACC
NcoI 1376 CCATGG C^CATGG
1525 CCATGG
SacI 2961 GAGCTC GAGCT^C
3799 GAGCTC
SgrAI 69 CGCCGGCG C[GA]^CCGG[CT]G
529 CGCCGGCG
SmlI 3458 CTCAAG C^T[CT][GA]AG
3742 CTCAAG
SpeI 2913 ACTAGT A^CTAGT
3502 ACTAGT
Tsp509I 1840 AATT ^AATT
3611 AATT
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII