##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
Alw44I      ApaLI       AscI        Asp718I     AspI        
AviII       AvrII       BbrPI       BcgI        BciVI       
BfrI        BglII       BlnI        BsaBI       BsaWI       
BseAI       BsiWI       BsmBI       BspEI       BsrDI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CpoI        DraI        DrdI        EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HpaI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SalI        
SbfI        ScaI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SwaI        Tth111I     
XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AatII         450  GACGTC               GACGT^C
AccI         2686  GTAGAC               GT^[AC][GT]AC
Aor51HI       636  AGCGCT               AGC^GCT
AseI          285  ATTAAT               AT^TAAT
AsnI          285  ATTAAT               AT^TAAT
Asp700I      1836  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        4057  GGATCC               G^GATCC
BclI          722  TGATCA               T^GATCA
BsaI          386  GAGACC               GGTCTC(1/5)
Bsp1407I     2669  TGTACA               T^GTACA
BspDI          18  ATCGAT               AT^CGAT
BspLU11I     2672  ACATGT               A^CATGT
BsrGI        2669  TGTACA               T^GTACA
BssHII       2585  GCGCGC               G^CGCGC
ClaI           18  ATCGAT               AT^CGAT
Eco47III      636  AGCGCT               AGC^GCT
FbaI          722  TGATCA               T^GATCA
HincII        808  GTCAAC               GT[CT]^[GA]AC
HindII        808  GTCAAC               GT[CT]^[GA]AC
HindIII       207  AAGCTT               A^AGCTT
NaeI         2344  GCCGGC               GCC^GGC
NcoI         2818  CCATGG               C^CATGG
NgoMIV       2344  GCCGGC               G^CCGGC
PshBI         285  ATTAAT               AT^TAAT
SapI         2985  GAAGAGC              GCTCTTC(1/4)
SfiI          900  GGCCGCGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI        2669  TGTACA               T^GTACA
SspI           29  AATATT               AAT^ATT
XbaI         1962  TCTAGA               T^CTAGA
XmnI         1836  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AcsI          151  GAATTT               [GA]^AATT[CT]
             1219  AAATTC              
AflIII       2672  ACATGT               A^C[GA][CT]GT
             3143  ACACGT              
ApoI          151  GAATTT               [GA]^AATT[CT]
             1219  AAATTC              
BsmI          497  GCATTC               GAATGC(1/-1)
             1958  GCATTC              
BspHI         725  TCATGA               T^CATGA
             4217  TCATGA              
BspMI        1141  ACCTGC               ACCTGC(4/8)
             3251  ACCTGC              
BsrFI        2344  GCCGGC               [GA]^CCGG[CT]
             3418  ACCGGC              
Cfr10I       2344  GCCGGC               [GA]^CCGG[CT]
             3418  ACCGGC              
RcaI          725  TCATGA               T^CATGA
             4217  TCATGA              
SmlI         2554  CTCAAG               C^T[CT][GA]AG
             3619  CTCAAG              
SphI         2861  GCATGC               GCATG^C
             3314  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  141 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AhdI        
AluI        AlwI        AlwNI       AocI        ApaI        
AspEI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI