Restriction map for
pF1KSDA0323
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbrPI BcgI
BciVI BfrI BglII BlnI BsaAI
BsiEI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Cfr10I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KspI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 1845 GGTACC G^GTACC
AccII 147 CGCG CG^CG
AcsI 1888 GAATTT [GA]^AATT[CT]
AlwNI 2044 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 981 CCTCAGG CC^T[ACGT]AGG
ApoI 1888 GAATTT [GA]^AATT[CT]
Asp718I 1845 GGTACC G^GTACC
BamHI 1386 GGATCC G^GATCC
BbvCI 1632 CCTCAGC CCTCAGC(-5/-2)
BclI 482 TGATCA T^GATCA
BglI 1566 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1788 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1262 GAGACG CGTCTC(1/5)
BspMI 1206 ACCTGC ACCTGC(4/8)
BssSI 1658 CACGAG CACGAG(-5/-1)
BstEII 1540 GGTCACC G^GT[ACGT]ACC
BstPI 1540 GGTCACC G^GT[ACGT]ACC
BstUI 147 CGCG CG^CG
BstXI 917 CCAGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 981 CCTCAGG CC^T[ACGT]AGG
Eco81I 981 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1540 GGTCACC G^GT[ACGT]ACC
FbaI 482 TGATCA T^GATCA
KpnI 1845 GGTACC GGTAC^C
MamI 1788 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2150 TTAA T^TAA
MslI 1512 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 147 CGCG CG^CG
PshAI 1534 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 529 GAAGAGC GCTCTTC(1/4)
SbfI 1380 CCTGCAGG CCTGCA^GG
ScaI 1523 AGTACT AGT^ACT
Sse8387I 1380 CCTGCAGG CCTGCA^GG
Tru9I 2150 TTAA T^TAA
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccIII 261 TCCGGA T^CCGGA
577 TCCGGA
ApaI 137 GGGCCC GGGCC^C
294 GGGCCC
BalI 1419 TGGCCA TGG^CCA
1463 TGGCCA
BbeI 465 GGCGCC GGCGC^C
1330 GGCGCC
BbsI 2013 GAAGAC GAAGAC(2/6)
1920 GTCTTC
BfaI 716 CTAG C^TAG
806 CTAG
BpuAI 2013 GAAGAC GAAGAC(2/6)
1920 GTCTTC
BsaI 1235 GAGACC GGTCTC(1/5)
1253 GAGACC
BseAI 261 TCCGGA T^CCGGA
577 TCCGGA
BsmI 1173 GAATGC GAATGC(1/-1)
2006 GCATTC
BspEI 261 TCCGGA T^CCGGA
577 TCCGGA
BsrBI 541 GAGCGG CCGCTC(-3/-3)
2029 GAGCGG
BsrDI 1506 CATTGC GCAATG(2/0)
1512 CATTGC
DrdI 1549 GACATAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1911 GACACAGGGGTC
EaeI 1419 TGGCCA [CT]^GGCC[GA]
1463 TGGCCA
KasI 465 GGCGCC G^GCGCC
1330 GGCGCC
MaeI 716 CTAG C^TAG
806 CTAG
MluNI 1419 TGGCCA TGG^CCA
1463 TGGCCA
MroI 261 TCCGGA T^CCGGA
577 TCCGGA
MscI 1419 TGGCCA TGG^CCA
1463 TGGCCA
NarI 465 GGCGCC GG^CGCC
1330 GGCGCC
PpuMI 843 GGGACCC [GA]G^G[AT]CC[CT]
1760 GGGACCT
PvuII 286 CAGCTG CAG^CTG
2044 CAGCTG
SacI 759 GAGCTC GAGCT^C
797 GAGCTC
SfoI 465 GGCGCC GGC^GCC
1330 GGCGCC
SmaI 633 CCCGGG CCC^GGG
2018 CCCGGG
Tsp509I 1737 AATT ^AATT
1889 AATT
XmaCI 633 CCCGGG C^CCGGG
2018 CCCGGG
XmaI 633 CCCGGG C^CCGGG
2018 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI XhoII