Restriction map for
pF1KSDA0334
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AvaI AviII AvrII BalI BbeI
BbrPI BbsI BbvCI BcgI BcnI
BfrI BglI BglII BlnI BpmI
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CfoI ClaI CpoI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KspI MluI MluNI MroI
MscI MvnI NarI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 2172 GGTACC G^GTACC
AciI 2344 CCGC CCGC(-3/-1)
Alw44I 798 GTGCAC G^TGCAC
ApaLI 798 GTGCAC G^TGCAC
AseI 336 ATTAAT AT^TAAT
AsnI 336 ATTAAT AT^TAAT
Asp700I 1657 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2172 GGTACC G^GTACC
AspI 2247 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 172 GGATCC G^GATCC
BclI 1272 TGATCA T^GATCA
BlpI 2326 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2326 GCTCAGC GC^T[ACGT]AGC
BsaBI 931 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 263 CTGCAC GTGCAG(16/14)
BsiEI 1328 CGGCCG CG[GA][CT]^CG
BsmBI 1332 CGTCTC CGTCTC(1/5)
BsmFI 1533 GTCCC GGGAC(10/14)
BsrFI 2489 GCCGGC [GA]^CCGG[CT]
BstAPI 978 GCACTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2326 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2489 GCCGGC [GA]^CCGG[CT]
EaeI 1328 CGGCCG [CT]^GGCC[GA]
EagI 1328 CGGCCG C^GGCCG
EclXI 1328 CGGCCG C^GGCCG
Eco52I 1328 CGGCCG C^GGCCG
EcoNI 1023 CCTGACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1272 TGATCA T^GATCA
KpnI 2172 GGTACC GGTAC^C
MaeII 135 ACGT A^CGT
MamI 931 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1585 CAATTG C^AATTG
MunI 1585 CAATTG C^AATTG
NaeI 2489 GCCGGC GCC^GGC
NgoMIV 2489 GCCGGC G^CCGGC
PflFI 2247 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 336 ATTAAT AT^TAAT
ScaI 2179 AGTACT AGT^ACT
SfaNI 274 GATGC GCATC(5/9)
Tth111I 2247 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1815 CCAGATTCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1657 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwNI 1193 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1681 CAGGGGCTG
AvaII 1671 GGTCC G^G[AT]CC
2025 GGTCC
BanI 876 GGCACC G^G[CT][GA]CC
2172 GGTACC
BanII 30 GAGCTC G[GA]GC[CT]^C
2280 GAGCTC
BciVI 1371 GGATAC GTATCC(6/5)
673 GTATCC
BstF5I 1764 CATCC GGATG(2/0)
945 GGATG
BstXI 1209 CCAAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1461 CCAGTCTGTTGG
BstYI 172 GGATCC [GA]^GATC[CT]
1361 AGATCC
DraI 552 TTTAAA TTT^AAA
663 TTTAAA
DsaI 1674 CCATGG C^C[GA][CT]GG
2376 CCATGG
EcoRI 574 GAATTC G^AATTC
1086 GAATTC
FokI 1764 CATCC GGATG(9/13)
945 GGATG
HapII 2015 CCGG C^CGG
2490 CCGG
HpaII 2015 CCGG C^CGG
2490 CCGG
MflI 172 GGATCC [GA]^GATC[CT]
1361 AGATCC
MslI 940 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1989 CACTATGGTG
MspA1I 1193 CAGCTG C[AC]G^C[GT]G
2329 CAGCTG
MspI 2015 CCGG C^CGG
2490 CCGG
NcoI 1674 CCATGG C^CATGG
2376 CCATGG
NspI 587 ACATGC [GA]CATG^[CT]
1529 GCATGT
PvuII 1193 CAGCTG CAG^CTG
2329 CAGCTG
SacI 30 GAGCTC GAGCT^C
2280 GAGCTC
SspI 548 AATATT AAT^ATT
1618 AATATT
TaqI 34 TCGA T^CGA
288 TCGA
TthHB8I 34 TCGA T^CGA
288 TCGA
XhoII 172 GGATCC [GA]^GATC[CT]
1361 AGATCC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BbvI BfaI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspHI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XbaI