Restriction map for
pF1KSDA0341
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbrPI
BciVI BclI BfrI BlnI BsaAI
BsaBI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrFI BsrGI BssHII
Bst1107I BstBI BstF5I BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FokI FseI HincII HindII HindIII
HpaI KpnI MaeII MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SexAI
SfaNI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 1538 ACATGT A^C[GA][CT]GT
Alw44I 1076 GTGCAC G^TGCAC
AlwI 1518 GATCC GGATC(4/5)
ApaLI 1076 GTGCAC G^TGCAC
Asp700I 375 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 992 GGCGCC GGCGC^C
BcgI 1293 GCAGGCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1190 AGATCT A^GATCT
BsmI 335 GCATTC GAATGC(1/-1)
BspLU11I 1538 ACATGT A^CATGT
BssSI 916 CACGAG CACGAG(-5/-1)
BstEII 921 GGTGACC G^GT[ACGT]ACC
BstPI 921 GGTGACC G^GT[ACGT]ACC
BstXI 120 CCAGCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 916 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 79 GAAGAG CTCTTC(1/4)
EcoO65I 921 GGTGACC G^GT[ACGT]ACC
KasI 992 GGCGCC G^GCGCC
Ksp632I 79 GAAGAG CTCTTC(1/4)
KspI 85 CCGCGG CCGC^GG
MseI 644 TTAA T^TAA
NarI 992 GGCGCC GG^CGCC
NcoI 32 CCATGG C^CATGG
NspI 1538 ACATGT [GA]CATG^[CT]
PaeR7I 1615 CTCGAG C^TCGAG
PpuMI 681 AGGACCC [GA]G^G[AT]CC[CT]
SacI 802 GAGCTC GAGCT^C
SacII 85 CCGCGG CCGC^GG
SapI 79 GAAGAGC GCTCTTC(1/4)
SbfI 1284 CCTGCAGG CCTGCA^GG
SfiI 489 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 992 GGCGCC GGC^GCC
Sse8387I 1284 CCTGCAGG CCTGCA^GG
TaqI 1616 TCGA T^CGA
Tru9I 644 TTAA T^TAA
TthHB8I 1616 TCGA T^CGA
XhoI 1615 CTCGAG C^TCGAG
XmnI 375 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 137 TCCGGA T^CCGGA
1032 TCCGGA
AcyI 402 GGCGTC G[GA]^CG[CT]C
992 GGCGCC
AviII 998 TGCGCA TGC^GCA
1277 TGCGCA
BalI 2 TGGCCA TGG^CCA
636 TGGCCA
BanI 992 GGCGCC G^G[CT][GA]CC
1051 GGCACC
BbsI 1116 GAAGAC GAAGAC(2/6)
1203 GAAGAC
BbvCI 165 CCTCAGC CCTCAGC(-5/-2)
522 CCTCAGC
BpuAI 1116 GAAGAC GAAGAC(2/6)
1203 GAAGAC
BsaHI 402 GGCGTC G[GA]^CG[CT]C
992 GGCGCC
BsaI 790 GAGACC GGTCTC(1/5)
104 GGTCTC
BsaWI 137 TCCGGA [AT]^CCGG[AT]
1032 TCCGGA
BseAI 137 TCCGGA T^CCGGA
1032 TCCGGA
BsmFI 405 GTCCC GGGAC(10/14)
1607 GTCCC
BspEI 137 TCCGGA T^CCGGA
1032 TCCGGA
BspMI 173 ACCTGC ACCTGC(4/8)
849 GCAGGT
BsrBI 907 GAGCGG CCGCTC(-3/-3)
1465 GAGCGG
BsrDI 27 CATTGC GCAATG(2/0)
434 GCAATG
BstYI 1190 AGATCT [GA]^GATC[CT]
1517 AGATCC
DsaI 32 CCATGG C^C[GA][CT]GG
85 CCGCGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
636 TGGCCA
FspI 998 TGCGCA TGC^GCA
1277 TGCGCA
HgaI 1246 GACGC GACGC(5/10)
403 GCGTC
Hin1I 402 GGCGTC G[GA]^CG[CT]C
992 GGCGCC
HphI 283 GGTGA GGTGA(8/7)
921 GGTGA
MaeIII 284 GTGAC ^GT[ACGT]AC
922 GTGAC
MflI 1190 AGATCT [GA]^GATC[CT]
1517 AGATCC
MluNI 2 TGGCCA TGG^CCA
636 TGGCCA
MroI 137 TCCGGA T^CCGGA
1032 TCCGGA
MscI 2 TGGCCA TGG^CCA
636 TGGCCA
Tsp45I 284 GTGAC ^GT[GC]AC
922 GTGAC
XcmI 32 CCATGGGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
389 CCATGCAAAGCCTGG
XhoII 1190 AGATCT [GA]^GATC[CT]
1517 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeI MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI SmaI SmlI StyI TseI
TspRI Van91I XmaCI XmaI