Restriction map for
pF1KE3703
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# NO CUT ENZYMES ( 71 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI AscI Asp718I AspEI
AspI AvrII BamHI BbsI BciVI
BfrI BlnI BpuAI BsaBI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EcoO65I EcoRV FseI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SfuI SgrAI SnaBI
SpeI SphI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 3412 GACGTC GACGT^C
AcsI 2050 GAATTC [GA]^AATT[CT]
Aor51HI 4255 AGCGCT AGC^GCT
ApoI 2050 GAATTC [GA]^AATT[CT]
AseI 4381 ATTAAT AT^TAAT
AsnI 4381 ATTAAT AT^TAAT
AviII 3374 TGCGCA TGC^GCA
BclI 4595 TGATCA T^GATCA
BsiEI 4973 CGGCCG CG[GA][CT]^CG
BspHI 2084 TCATGA T^CATGA
BspMI 3056 ACCTGC ACCTGC(4/8)
EagI 4973 CGGCCG C^GGCCG
EclXI 4973 CGGCCG C^GGCCG
Eco47III 4255 AGCGCT AGC^GCT
Eco52I 4973 CGGCCG C^GGCCG
EcoNI 1981 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2050 GAATTC G^AATTC
FbaI 4595 TGATCA T^GATCA
FspI 3374 TGCGCA TGC^GCA
HgaI 4885 GACGC GACGC(5/10)
HincII 4186 GTCAAC GT[CT]^[GA]AC
HindII 4186 GTCAAC GT[CT]^[GA]AC
NaeI 4998 GCCGGC GCC^GGC
NgoMIV 4998 GCCGGC G^CCGGC
PshBI 4381 ATTAAT AT^TAAT
RcaI 2084 TCATGA T^CATGA
SbfI 4827 CCTGCAGG CCTGCA^GG
ScaI 2435 AGTACT AGT^ACT
SexAI 4784 ACCTGGT A^CC[AT]GGT
SfiI 3111 GGCCTCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 544 CTTGAG C^T[CT][GA]AG
Sse8387I 4827 CCTGCAGG CCTGCA^GG
SspI 4333 AATATT AAT^ATT
Tsp509I 2051 AATT ^AATT
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# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 4538 ACGTGT A^C[GA][CT]GT
4778 ACACGT
Alw44I 4228 GTGCAC G^TGCAC
4396 GTGCAC
ApaLI 4228 GTGCAC G^TGCAC
4396 GTGCAC
Asp700I 1329 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2101 GAATCCATTC
BbrPI 4084 CACGTG CAC^GTG
4537 CACGTG
BbvCI 666 CCTCAGC CCTCAGC(-5/-2)
2543 CCTCAGC
BcgI 4797 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3977 GCACCAACATCG
BglII 1487 AGATCT A^GATCT
3782 AGATCT
BsmI 3432 GAATGC GAATGC(1/-1)
1244 GCATTC
Bsp1407I 3604 TGTACA T^GTACA
4124 TGTACA
BsrBI 2767 GAGCGG CCGCTC(-3/-3)
3364 GAGCGG
BsrGI 3604 TGTACA T^GTACA
4124 TGTACA
BssHII 3420 GCGCGC G^CGCGC
4915 GCGCGC
BssSI 4231 CACGAG CACGAG(-5/-1)
4372 CTCGTG
EcoT22I 1 ATGCAT ATGCA^T
4325 ATGCAT
HindIII 414 AAGCTT A^AGCTT
1851 AAGCTT
MseI 4382 TTAA T^TAA
4739 TTAA
NruI 1554 TCGCGA TCG^CGA
5032 TCGCGA
NsiI 1 ATGCAT ATGCA^T
4325 ATGCAT
NspI 3254 ACATGC [GA]CATG^[CT]
4073 ACATGC
PflMI 969 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4412 CCACCGGCTGG
PmaCI 4084 CACGTG CAC^GTG
4537 CACGTG
PmlI 4084 CACGTG CAC^GTG
4537 CACGTG
SspBI 3604 TGTACA T^GTACA
4124 TGTACA
Tru9I 4382 TTAA T^TAA
4739 TTAA
Van91I 969 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4412 CCACCGGCTGG
XmnI 1329 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2101 GAATCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI