Restriction map for
pF1KSDA0360
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AvrII
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmBI Bsp1407I BspDI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MunI NaeI NarI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 60 CGCG CG^CG
AccIII 2129 TCCGGA T^CCGGA
AflIII 1284 ACATGT A^C[GA][CT]GT
Alw44I 2024 GTGCAC G^TGCAC
ApaLI 2024 GTGCAC G^TGCAC
Asp700I 2091 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1324 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 118 TGCGCA TGC^GCA
BamHI 324 GGATCC G^GATCC
BanI 652 GGTGCC G^G[CT][GA]CC
BseAI 2129 TCCGGA T^CCGGA
BsmI 958 GCATTC GAATGC(1/-1)
BspEI 2129 TCCGGA T^CCGGA
BspHI 1538 TCATGA T^CATGA
BspLU11I 1284 ACATGT A^CATGT
BspMI 615 GCAGGT ACCTGC(4/8)
BstAPI 1447 GCACTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1818 GGTGACC G^GT[ACGT]ACC
BstPI 1818 GGTGACC G^GT[ACGT]ACC
BstUI 60 CGCG CG^CG
DraIII 1448 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1818 GGTGACC G^GT[ACGT]ACC
FspI 118 TGCGCA TGC^GCA
HgaI 61 GCGTC GACGC(5/10)
MroI 2129 TCCGGA T^CCGGA
MseI 34 TTAA T^TAA
MvnI 60 CGCG CG^CG
NcoI 2913 CCATGG C^CATGG
NdeI 2872 CATATG CA^TATG
NheI 79 GCTAGC G^CTAGC
PflFI 1324 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1538 TCATGA T^CATGA
SbfI 2685 CCTGCAGG CCTGCA^GG
SexAI 2891 ACCAGGT A^CC[AT]GGT
SmaI 2060 CCCGGG CCC^GGG
SphI 1640 GCATGC GCATG^C
Sse8387I 2685 CCTGCAGG CCTGCA^GG
SspI 2926 AATATT AAT^ATT
TaqI 466 TCGA T^CGA
Tru9I 34 TTAA T^TAA
Tth111I 1324 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 466 TCGA T^CGA
XmaCI 2060 CCCGGG C^CCGGG
XmaI 2060 CCCGGG C^CCGGG
XmnI 2091 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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AcsI 142 GAATTC [GA]^AATT[CT]
2070 GAATTC
ApaI 336 GGGCCC GGGCC^C
1931 GGGCCC
ApoI 142 GAATTC [GA]^AATT[CT]
2070 GAATTC
BsrBI 2812 CCGCTC CCGCTC(-3/-3)
2851 GAGCGG
BsrDI 1208 GCAATG GCAATG(2/0)
2695 GCAATG
BsrFI 1262 ACCGGC [GA]^CCGG[CT]
1799 ACCGGC
Cfr10I 1262 ACCGGC [GA]^CCGG[CT]
1799 ACCGGC
EcoNI 679 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2685 CCTGCAGGAGG
EcoRI 142 GAATTC G^AATTC
2070 GAATTC
FauI 410 GCGGG CCCGC(4/6)
2853 GCGGG
SapI 550 GAAGAGC GCTCTTC(1/4)
2814 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BalI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII