Restriction map for
pF1KSDA0364
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbrPI BclI BfrI BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FbaI FseI
FspI HindIII KpnI KspI MamI
MluI MroI NaeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1432 CGCG CG^CG
AcyI 1915 GGCGCC G[GA]^CG[CT]C
AgeI 284 ACCGGT A^CCGGT
Alw44I 3741 GTGCAC G^TGCAC
AocI 544 CCTGAGG CC^T[ACGT]AGG
ApaI 676 GGGCCC GGGCC^C
ApaLI 3741 GTGCAC G^TGCAC
AspI 3601 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2351 CCCGAG C^[CT]CG[GA]G
BbeI 1915 GGCGCC GGCGC^C
BbvCI 3577 CCTCAGC CCTCAGC(-5/-2)
BcgI 2675 GCATGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2968 GGATAC GTATCC(6/5)
BglI 1814 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3431 AGATCT A^GATCT
BlpI 2896 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2896 GCTGAGC GC^T[ACGT]AGC
BsaHI 1915 GGCGCC G[GA]^CG[CT]C
BseRI 1963 GAGGAG GAGGAG(10/8)
BsoBI 2351 CCCGAG C^[CT]CG[GA]G
BspDI 1084 ATCGAT AT^CGAT
BsrBI 395 CCGCTC CCGCTC(-3/-3)
BssSI 2569 CTCGTG CACGAG(-5/-1)
BstUI 1432 CGCG CG^CG
Bsu36I 544 CCTGAGG CC^T[ACGT]AGG
CelII 2896 GCTGAGC GC^T[ACGT]AGC
ClaI 1084 ATCGAT AT^CGAT
DraI 463 TTTAAA TTT^AAA
DraIII 594 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 544 CCTGAGG CC^T[ACGT]AGG
EcoRI 2581 GAATTC G^AATTC
FauI 394 CCCGC CCCGC(4/6)
HaeII 1915 GGCGCC [GA]GCGC^[CT]
HgaI 871 GACGC GACGC(5/10)
Hin1I 1915 GGCGCC G[GA]^CG[CT]C
HpaI 415 GTTAAC GTT^AAC
KasI 1915 GGCGCC G^GCGCC
MfeI 2364 CAATTG C^AATTG
MunI 2364 CAATTG C^AATTG
MvnI 1432 CGCG CG^CG
NarI 1915 GGCGCC GG^CGCC
NdeI 2386 CATATG CA^TATG
NheI 3807 GCTAGC G^CTAGC
PflFI 3601 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 284 ACCGGT A^CCGGT
SacI 2566 GAGCTC GAGCT^C
ScaI 1311 AGTACT AGT^ACT
SfoI 1915 GGCGCC GGC^GCC
StuI 3665 AGGCCT AGG^CCT
Tth111I 3601 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AspHI 2566 GAGCTC G[AT]GC[AT]^C
3741 GTGCAC
BalI 515 TGGCCA TGG^CCA
1249 TGGCCA
BamHI 209 GGATCC G^GATCC
3016 GGATCC
BsiHKAI 2566 GAGCTC G[AT]GC[AT]^C
3741 GTGCAC
BsmI 65 GCATTC GAATGC(1/-1)
3037 GCATTC
BspMI 274 GCAGGT ACCTGC(4/8)
1890 GCAGGT
BsrDI 3879 CATTGC GCAATG(2/0)
1697 GCAATG
BsrFI 284 ACCGGT [GA]^CCGG[CT]
2952 GCCGGT
BstEII 1650 GGTCACC G^GT[ACGT]ACC
2337 GGTCACC
BstPI 1650 GGTCACC G^GT[ACGT]ACC
2337 GGTCACC
CfoI 1916 GCGC GCG^C
2652 GCGC
Cfr10I 284 ACCGGT [GA]^CCGG[CT]
2952 GCCGGT
DrdI 306 GACAGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2811 GACAACCATGTC
EaeI 515 TGGCCA [CT]^GGCC[GA]
1249 TGGCCA
EcoO65I 1650 GGTCACC G^GT[ACGT]ACC
2337 GGTCACC
EcoRV 162 GATATC GAT^ATC
3676 GATATC
HhaI 1916 GCGC GCG^C
2652 GCGC
HinP1I 1916 GCGC G^CGC
2652 GCGC
HincII 415 GTTAAC GT[CT]^[GA]AC
1393 GTTGAC
HindII 415 GTTAAC GT[CT]^[GA]AC
1393 GTTGAC
MaeII 1517 ACGT A^CGT
3617 ACGT
MluNI 515 TGGCCA TGG^CCA
1249 TGGCCA
MscI 515 TGGCCA TGG^CCA
1249 TGGCCA
PstI 439 CTGCAG CTGCA^G
3648 CTGCAG
SapI 397 GCTCTTC GCTCTTC(1/4)
1659 GCTCTTC
SmlI 1643 CTCAAG C^T[CT][GA]AG
1996 CTTGAG
XbaI 333 TCTAGA T^CTAGA
3552 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI