Restriction map for
pF1KSDA0379
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AspI AvaII AviII
AvrII BbeI BbrPI BbvCI BcnI
BglI BlnI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
FauI FseI FspI HaeII HhaI
HinP1I KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 378 GGTACC G^GTACC
AccI 1223 GTCTAC GT^[AC][GT]AC
AflII 1140 CTTAAG C^TTAAG
AflIII 2308 ACATGT A^C[GA][CT]GT
AocI 1607 CCTCAGG CC^T[ACGT]AGG
Asp700I 262 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 378 GGTACC G^GTACC
AvaI 305 CTCGAG C^[CT]CG[GA]G
BamHI 1496 GGATCC G^GATCC
BbsI 1559 GTCTTC GAAGAC(2/6)
BcgI 2400 CGAAGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1513 GGATAC GTATCC(6/5)
BfrI 1140 CTTAAG C^TTAAG
BlpI 2526 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2526 GCTCAGC GC^T[ACGT]AGC
BpuAI 1559 GTCTTC GAAGAC(2/6)
BsaI 3151 GAGACC GGTCTC(1/5)
BsoBI 305 CTCGAG C^[CT]CG[GA]G
BspLU11I 2308 ACATGT A^CATGT
BssSI 2302 CACGAG CACGAG(-5/-1)
Bsu36I 1607 CCTCAGG CC^T[ACGT]AGG
CelII 2526 GCTCAGC GC^T[ACGT]AGC
DsaI 945 CCACGG C^C[GA][CT]GG
Eco81I 1607 CCTCAGG CC^T[ACGT]AGG
EcoT14I 150 CCTTGG C^C[AT][AT]GG
EcoT22I 2108 ATGCAT ATGCA^T
HapII 2157 CCGG C^CGG
HpaI 100 GTTAAC GTT^AAC
HpaII 2157 CCGG C^CGG
KpnI 378 GGTACC GGTAC^C
MspI 2157 CCGG C^CGG
NsiI 2108 ATGCAT ATGCA^T
PaeR7I 305 CTCGAG C^TCGAG
SacI 3130 GAGCTC GAGCT^C
ScaI 3107 AGTACT AGT^ACT
SpeI 2710 ACTAGT A^CTAGT
StyI 150 CCTTGG C^C[AT][AT]GG
XcmI 2903 CCAATAAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 305 CTCGAG C^TCGAG
XmnI 262 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 1248 GAATTT [GA]^AATT[CT]
1306 AAATTT
AcyI 1962 GACGCC G[GA]^CG[CT]C
2160 GACGCC
AlwNI 1354 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2518 CAGCTGCTG
ApoI 1248 GAATTT [GA]^AATT[CT]
1306 AAATTT
BalI 1047 TGGCCA TGG^CCA
2939 TGGCCA
BanII 2207 GAGCCC G[GA]GC[CT]^C
3130 GAGCTC
BclI 653 TGATCA T^GATCA
1061 TGATCA
BpmI 188 CTGGAG CTGGAG(16/14)
1241 CTGGAG
BsaBI 1483 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2236 GATGCAAATC
BsaHI 1962 GACGCC G[GA]^CG[CT]C
2160 GACGCC
BseRI 1583 CTCCTC GAGGAG(10/8)
2474 CTCCTC
BspHI 2703 TCATGA T^CATGA
3050 TCATGA
BstEII 1546 GGTCACC G^GT[ACGT]ACC
1681 GGTCACC
BstPI 1546 GGTCACC G^GT[ACGT]ACC
1681 GGTCACC
EaeI 1047 TGGCCA [CT]^GGCC[GA]
2939 TGGCCA
EcoO65I 1546 GGTCACC G^GT[ACGT]ACC
1681 GGTCACC
FbaI 653 TGATCA T^GATCA
1061 TGATCA
HgaI 1962 GACGC GACGC(5/10)
2160 GACGC
Hin1I 1962 GACGCC G[GA]^CG[CT]C
2160 GACGCC
HincII 100 GTTAAC GT[CT]^[GA]AC
222 GTTGAC
HindII 100 GTTAAC GT[CT]^[GA]AC
222 GTTGAC
HindIII 371 AAGCTT A^AGCTT
3038 AAGCTT
MaeII 401 ACGT A^CGT
3122 ACGT
MamI 1483 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2236 GATGCAAATC
MluNI 1047 TGGCCA TGG^CCA
2939 TGGCCA
MscI 1047 TGGCCA TGG^CCA
2939 TGGCCA
PleI 2557 GACTC GAGTC(4/5)
3139 GACTC
RcaI 2703 TCATGA T^CATGA
3050 TCATGA
SspI 81 AATATT AAT^ATT
493 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AseI
AsnI AspHI BanI BbvI BfaI
BglII BmyI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII