Restriction map for
pF1KSDA0380
##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI AvrII BcgI
BciVI BfrI BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SfuI SgrAI SnaBI SpeI SspBI
StuI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 3186 GACGTC GACGT^C
AcsI 3879 AAATTC [GA]^AATT[CT]
AflIII 3353 ACGTGT A^C[GA][CT]GT
AgeI 832 ACCGGT A^CCGGT
AhdI 117 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3879 AAATTC [GA]^AATT[CT]
AspEI 117 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2249 TGCGCA TGC^GCA
BbeI 334 GGCGCC GGCGC^C
BbrPI 2953 CACGTG CAC^GTG
BglII 3164 AGATCT A^GATCT
BsaAI 2953 CACGTG [CT]AC^GT[GA]
BsmI 3237 GAATGC GAATGC(1/-1)
BsrDI 1688 GCAATG GCAATG(2/0)
BstAPI 207 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 117 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 2249 TGCGCA TGC^GCA
KasI 334 GGCGCC G^GCGCC
NaeI 629 GCCGGC GCC^GGC
NarI 334 GGCGCC GG^CGCC
NgoMIV 629 GCCGGC G^CCGGC
PinAI 832 ACCGGT A^CCGGT
PmaCI 2953 CACGTG CAC^GTG
PmlI 2953 CACGTG CAC^GTG
SbfI 4168 CCTGCAGG CCTGCA^GG
SfiI 777 GGCCAGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 334 GGCGCC GGC^GCC
SphI 4091 GCATGC GCATG^C
Sse8387I 4168 CCTGCAGG CCTGCA^GG
SspI 1096 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 1166 CGCG CG^CG
4551 CGCG
AfaI 727 GTAC GT^AC
2652 GTAC
Asp700I 682 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1095 GAATATTTTC
BamHI 510 GGATCC G^GATCC
1451 GGATCC
BclI 323 TGATCA T^GATCA
770 TGATCA
BsiEI 258 CGACCG CG[GA][CT]^CG
1766 CGACCG
BstUI 1166 CGCG CG^CG
4551 CGCG
EcoNI 2085 CCTCCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3435 CCTGCTAGAGG
FbaI 323 TGATCA T^GATCA
770 TGATCA
HincII 274 GTCAAC GT[CT]^[GA]AC
1909 GTTGAC
HindII 274 GTCAAC GT[CT]^[GA]AC
1909 GTTGAC
MseI 34 TTAA T^TAA
868 TTAA
MvnI 1166 CGCG CG^CG
4551 CGCG
NspI 528 ACATGC [GA]CATG^[CT]
4091 GCATGC
PshAI 1006 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3835 GACATGGGTC
RsaI 727 GTAC GT^AC
2652 GTAC
SacI 419 GAGCTC GAGCT^C
3214 GAGCTC
SexAI 1981 ACCAGGT A^CC[AT]GGT
3428 ACCAGGT
Tru9I 34 TTAA T^TAA
868 TTAA
XcmI 2843 CCAGCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4023 CCAGTCAGAGCCTGG
XmnI 682 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1095 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI